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SAE1 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115307

SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to… More details

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Product Overview

Online Datasheet in PDF
Product Name
SAE1, Cell Lysate
Full Product Name
Human SAE1 Baculovirus-Insect cells Overexpression Lysate
Product Gene Name
SAE1 cell lysate
Product Synonym Names
Human SAE1 Insect Cell Lysate (WB positive control); SUMO1 activating enzyme subunit 1; Human AOS1 Overexpression Lysate; Human HSPC140 Overexpression Lysate; Human SUA1 Overexpression Lysate; Human UBLE1A Overexpression Lysate
Sequence
Met1-Lys34 6
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
38,450 Da
Sequence Positions
348aa
Chromosome Location
Chromosome: 19; NC_000019.9 (47634080..47713893). Location: 19q13.32
OMIM
613294
3D Structure
ModBase 3D Structure for Q9UBE0
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was expressed and purified.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for SAE1 cell lysate
Western Blot (WB)
Application Notes for SAE1 cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for SAE1 cell lysate

Gong L, et al. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448(1):185-9.Lois LM, et al. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. EMBO J. 24(3):439-51.Tatham MH, et al. (2001) Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J Biol Chem. 276(38):35368-74.

Other Notes

Small volumes of SAE1 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAE1 purchase

MBS8115307 is a cell lysate targeting SAE1. Also known as Human SAE1 Baculovirus-Insect cells Overexpression Lysate. Host: Baculovirus-Insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #
225543285
NCBI GeneID
10055
NCBI Accession #
NP_001139185.1
UniProt Primary Accession #
Q9UBE0
UniProt Secondary Accession #
O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6
UniProt Related Accession #
Q9UBE0
GenBank Nucleotide #
NM_001145713.1
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform b
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1A
Protein Family
SUMO-activating enzyme
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
UniProt Entry Name
SAE1_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for SAE1

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytosol; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification
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Research Articles on SAE1

1. Data indicate the role of anti-SUMO activating enzyme SAE1 and SAE2 antibody as marker of dermatomyositis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SAE1 cell lysate

 Reference Product  PubMed Publications
 UBA2 cell lysate  >17 publications with SAE1 and UBA2
 SUMO2 cell lysate  >3 publications with SAE1 and SUMO2
 SUMO3 cell lysate  >2 publications with SAE1 and SUMO3

Pathways associated with SAE1 cell lysate

 Products by Pathway  Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with SAE1 cell lysate

 Disease Name  Pubmed Publications
 Breast Diseases Antibodies  >2 publications with SAE1 and Breast Diseases
 Disease Models, Animal Antibodies  >2 publications with SAE1 and Disease Models, Animal
 Abnormalities, Multiple Antibodies  >1 publications with SAE1 and Abnormalities, Multiple
 Muscular Diseases Antibodies  >1 publications with SAE1 and Muscular Diseases
 Brain Diseases Antibodies  >1 publications with SAE1 and Brain Diseases
 Death Antibodies  >1 publications with SAE1 and Death

Organs/Tissues associated with SAE1 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SAE1 and Brain
 Muscle Antibodies  >2 publications with SAE1 and Muscle
 Skin Antibodies  >2 publications with SAE1 and Skin
 Vascular Antibodies  >1 publications with SAE1 and Vascular
 Connective Tissue Antibodies  >1 publications with SAE1 and Connective Tissue
 Blood Antibodies  >1 publications with SAE1 and Blood
 Kidney Antibodies  >1 publications with SAE1 and Kidney
 Uterus Antibodies  >1 publications with SAE1 and Uterus
 Embryonic Tissue Antibodies  >1 publications with SAE1 and Embryonic Tissue
 Lung Antibodies  >1 publications with SAE1 and Lung
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