Carboxylesterase 1 (CES1) Active Protein

Name: CES1 Active Protein; Esterase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS173123
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS173123

This human CES1 (carboxylesterase 1) active protein catalyzes hydrolysis of aromatic and aliphatic esters, supporting drug-screening and urinalysis spiking-control applications. Human esterase (CES 1), a prominently expressed carboxylesterase in most tissues,… More details

$245 / 5000 Units +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Esterase (CES1), Active Protein

Full Product Name

Esterase (PLE), Porcine Liver - High Purity

Product Gene Name

CES1 active protein

Product Synonym Gene Name

APLE; PMPMEase; REH; TGH

Product Synonym Names

Carboxylesterase, PLE

Host

Porcine Liver

Form/Format

Powder, lyophilized from 20 mM sodium phosphate, pH 7.5

Preparation and Storage

Ships on blue ice. Store at -20° C. Protect from moisture. Aliquot and freeze at -20°C after reconstitution.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

CAS Number

9016-18-6

EC Number

3.1.1.1

Appearance

Tan powder

Solubility

Clear to slightly hazy, straw to light yellow solution, may contain a small volume of precipitates (1 mg/mL saline).

Activity

120 U/mg

Unit Definition

One unit will catalyze the hydrolysis of one micromole of ethyl butyrate to butyrate and ethanol per minute at pH 8.0 and 37°C.

Protein

0.8 mg protein/mg (Coomasie)

Specific Activity

150 u/mg protein

Ammonia

< 0.01 micromole/mg

Reconstitution

1 mg/ml tris buffered saline. Reconsitute into provided container and centrifuge to ensure full recovery.

Handling

Handle as a potentially hazardous substance.

Product Categories/Family for CES1 active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Esterase (ple)

Other Notes

Small volumes of CES1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CES1 purchase

Human esterase (CES 1), a prominently expressed carboxylesterase in most tissues, catalyzes hydrolysis of aromatic and aliphatic esters. In research and in clinical practice, it can be used as a spiking control during drug screens and urinanlysis and as a catalyst in organic synthesis reactions. Evidence is also mounting - see Pubmed Europe database abstracts - for multiple enzyme target proteins and pathways. MyBiosource.com is the largest supplier of highly purified protein from porcine liver. High quality, protein sequence verified in combination with excellent enzymatic activity, make this product a top choice in Europe and worldwide. Applications are endless but include complex combination experimental computational enzymatic and biochemical assays and enzyme inhibitor drug screens in cells. Many of these protocols are freely available as abstract cited works in Europe PMC collections.

Additional Details

NCBI/Uniprot data below describe general gene information for CES1. It may not necessarily be applicable to this product.

NCBI GI #

153256

NCBI GeneID

397478

NCBI Accession #

AAA26744.1

UniProt Secondary Accession #

O97582; P82128

UniProt Related Accession #

Q29550

NCBI Official Full Name

esterase

NCBI Official Symbol

CES1

NCBI Official Synonym Symbols

REH; TGH; APLE; PMPMEase

UniProt Protein Name

Liver carboxylesterase

UniProt Synonym Protein Names

Proline-beta-naphthylamidase; Retinyl ester hydrolase; REH

Protein Family

Liver carboxylesterase

UniProt Gene Name

REH

UniProt Synonym Gene Names

REH

UniProt Entry Name

EST1_PIG

NCBI Protein Information

liver carboxylesterase; retinyl ester hydrolase; triacylglycerol hydrolase; proline-beta-naphthylamidase; alternative pig liver esterase; prenylated methylated protein methyl esterase; carboxylesterase 1 (monocyte/macrophage serine esterase 1)

UniProt Comments for CES1

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Active towards triacylglycerides containing short-chain fatty acids from C2 to C6, and 13-monoacylglycerols containing fatty acids from C2 to C12. Inactive on long-chain triacylglycerols and diacylglycerol. Hydrolyzes aromatic and alkyl esters and vitamin A acetate. The hydrolysis rate depends upon the amino acid promoiety and the esterification site of the prodrug. Aromatic promoieties are favored, highest rates are observed with phenylalanyl progdrugs, hydrolysis of valyl and isoleucyl prodrugs is less efficient. With floxuridine prodrugs, activity is higher on 5' monoesters than on 3' monoesters. With gemcitabine prodrugs, activity is higher on 3' monoesters than on 5' monoesters.

Research Articles on CES1

1. The objective of this study is to explore the mechanisms that regulate TGH expression in porcine adipocyte and its role in fasting-induced and Isoproterenol-stimulated lipolysis in porcine primary adipocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CES1 active protein

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram