CD320 Antigen (CD320) ELISA Kit for Chicken

Intended Uses: This CD320 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CD320. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CD320 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD320 antibody and an CD320-HRP conjugate. The assay sample and buffer are incubated together with CD320-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD320 concentration since CD320 from samples and CD320-HRP conjugate compete for the anti-CD320 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD320 from the sample, fewer sites are left to bind CD320-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD320 concentration in each sample is interpolated from this standard curve.

Small volumes of CD320 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7209454 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD320 antigen (CD320) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD320. The ELISA analytical biochemical technique of the MBS7209454 kit is based on CD320 antibody-CD320 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD320 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD320. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).