• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

M-CSF (CSF1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8123137

Background: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte/macrophage colony-stimulating factors are cytokines… More details

$290 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
M-CSF (CSF1), Active Protein
Full Product Name
M-CSF Protein, Mouse, Recombinant (ECD)
Product Gene Name
CSF1 active protein
Product Synonym Names
Mouse M-CSF/CSF-1 Protein (ECD); C87615 Protein, Mouse; Csfm Protein, Mouse; MCSF Protein, Mouse; op Protein, Mouse; colony stimulating factor 1 (macrophage)
Sequence
Met1-Glu26 2
Host
HEK293 Cells
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Purity/Purification
>95% as determined by SDS-PAGE
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Molecular Weight
29,353 Da
3D Structure
ModBase 3D Structure for P07141
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Lys 33
Tag
Native
Protein Construction
A DNA sequence encoding the mouse CSF1 (P07141-1)(Met1-Glu262) was expressed.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Immobilized mouse CSF1 at 2ug/ml (100uL/well) can bind mouse CSF1R-Fch. The EC50 of mouse CSF1R-Fch is 60-220ng/mL.
2. Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.8-4ng/mL.

SDS-Page of CSF1 active protein

CSF1 Active Protein – Cat# MBS8123137

Testing Data of CSF1 active protein

Immobilized mouse CSF1 at 2 ug/ml (100 ul/well) can bind mouse CSF1R-Fch, The EC50 of mouse CSF1R-Fch is 60-220 ng/mL.
CSF1 Active Protein, Testing Data – Cat# MBS8123137

Product References and Citations for CSF1 active protein

Pandit J. et al., 1992, Science. 258: 1358-62. Tokai M. et al., 2000, J Bacteriol. 182 (10): 2865-8. Fan X. et al., 2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11. Frangogiannis NG. et al., 2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92. Cupp JS. et al., 2007, Am J Surg Pathol. 31 (6): 970-6.

Other Notes

Small volumes of CSF1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSF1 purchase

MBS8123137 is an active protein targeting CSF1. Also known as M-CSF Protein, Mouse, Recombinant (ECD). Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF1. It may not necessarily be applicable to this product.

NCBI GI #
166064046
NCBI GeneID
12977
NCBI Accession #
NP_001107001.1
UniProt Primary Accession #
P07141
UniProt Secondary Accession #
Q3U395; Q8R3C8
UniProt Related Accession #
P07141
GenBank Nucleotide #
NM_001113529.1
NCBI Official Full Name
macrophage colony-stimulating factor 1 isoform 2
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
Csf1
NCBI Official Synonym Symbols
op; Csfm; MCSF; C87615
UniProt Protein Name
Macrophage colony-stimulating factor 1
Protein Family
Macrophage colony-stimulating factor
UniProt Gene Name
Csf1
UniProt Synonym Gene Names
Csfm; CSF-1; MCSF
UniProt Entry Name
CSF1_MOUSE
NCBI Protein Information
macrophage colony-stimulating factor 1; osteopetrosis

UniProt Comments for CSF1

M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; immune system process; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; positive regulation of Ras protein signal transduction; inflammatory response; positive regulation of macrophage differentiation; positive regulation of cell-matrix adhesion; regulation of ossification; positive regulation of cell migration
Show more

Research Articles on CSF1

1. CSF-1 has a role in macrophage mediation of chronic graft-versus-host disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CSF1 active protein

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram

Diseases associated with CSF1 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >38 publications with CSF1 and Inflammation
 Carcinoma Antibodies  >26 publications with CSF1 and Carcinoma
 Necrosis Antibodies  >21 publications with CSF1 and Necrosis
 Breast Neoplasms Antibodies  >21 publications with CSF1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >14 publications with CSF1 and Adenocarcinoma
 Lung Neoplasms Antibodies  >14 publications with CSF1 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >10 publications with CSF1 and Neoplasms, Experimental
 Lung Diseases Antibodies  >9 publications with CSF1 and Lung Diseases
 Liver Diseases Antibodies  >7 publications with CSF1 and Liver Diseases
 Osteitis Deformans Antibodies  >6 publications with CSF1 and Osteitis Deformans

Organs/Tissues associated with CSF1 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >70 publications with CSF1 and Blood
 Bone Antibodies  >61 publications with CSF1 and Bone
 Bone Marrow Antibodies  >34 publications with CSF1 and Bone Marrow
 Lung Antibodies  >28 publications with CSF1 and Lung
 Brain Antibodies  >16 publications with CSF1 and Brain
 Connective Tissue Antibodies  >13 publications with CSF1 and Connective Tissue
 Lymph Node Antibodies  >12 publications with CSF1 and Lymph Node
 Kidney Antibodies  >10 publications with CSF1 and Kidney
 Intestine Antibodies  >10 publications with CSF1 and Intestine
 Spleen Antibodies  >9 publications with CSF1 and Spleen
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000