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PD-L1 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488962

The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a… More details

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Product Overview

Online Datasheet in PDF
Product Name
PD-L1, Antibody
Full Product Name
Anti-PD-L1 [332M7], Rabbit IgG
Product Gene Name
anti-PD-L1 antibody
Product Synonym Names
Anti-PD-L1 [332M7]; CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1
Isotype
Rabbit IgG, Kappa
Clone Number
332M7
Host
Rabbit
Species Reactivity
Human, Mouse, Cynomolgus Monkey
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors.. PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD-L1 can mediate cancer immune evasion. Blocking this pathway, therefore provides a rationale for cancer immunotherapy.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
33,275 Da
OMIM
605402
3D Structure
ModBase 3D Structure for Q9NZQ7
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human PD-L1.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for anti-PD-L1 antibody

cancer; oncology; tumor
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PD-L1 antibody
Flow Cytometry (FC/FACS), Blocking (BL)

Flow Cytometry (FC/FACS) of anti-PD-L1 antibody

Flow cytometry using the Anti-PD-L1 antibody 332M7 (MBS488962). Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 332M7 (MBS488962-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Rabbit Anti-PD-L1 Antibody – Cat# MBS488962

Immunofluorescence (IF) of anti-PD-L1 antibody

Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 (MBS488962) 332M7 Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (MBS488962-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488962-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Rabbit Anti-PD-L1 Antibody, Immunofluorescence (IF) – Cat# MBS488962

Product References and Citations for anti-PD-L1 antibody

US20180346571 PMID:

Other Notes

Small volumes of anti-PD-L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PD-L1 purchase

MBS488962 is an antibody targeting PD-L1. Also known as Anti-PD-L1 [332M7], Rabbit IgG. Reactive in Human, Mouse, Cynomolgus Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PD-L1. It may not necessarily be applicable to this product.

NCBI GI #
390979639
NCBI GeneID
29126
NCBI Accession #
NP_001254635.1
UniProt Primary Accession #
Q9NZQ7
UniProt Secondary Accession #
Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
UniProt Related Accession #
Q9NZQ7
GenBank Nucleotide #
NM_001267706.1
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Synonym Protein Names
B7 homolog 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1

UniProt Comments for PD-L1

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction
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Research Articles on PD-L1

1. Upregulation of B7-H1 in gastric cancer tissue correlated with disease progression and prognosis of gastric cancer patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PD-L1 antibody

 Reference Product  PubMed Publications
 CD80 antibody  >381 publications with PD-L1 and CD80
 PDCD1 antibody  >300 publications with PD-L1 and PDCD1
 STAT1 antibody  >10 publications with PD-L1 and STAT1
 PTEN antibody  >7 publications with PD-L1 and PTEN

Pathways associated with anti-PD-L1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram

Diseases associated with anti-PD-L1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >328 publications with PD-L1 and Neoplasms
 Inflammation Antibodies  >102 publications with PD-L1 and Inflammation
 Liver Diseases Antibodies  >83 publications with PD-L1 and Liver Diseases
 Necrosis Antibodies  >70 publications with PD-L1 and Necrosis
 Nervous System Diseases Antibodies  >68 publications with PD-L1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >60 publications with PD-L1 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >58 publications with PD-L1 and Adenocarcinoma
 Kidney Diseases Antibodies  >41 publications with PD-L1 and Kidney Diseases
 Lung Diseases Antibodies  >38 publications with PD-L1 and Lung Diseases
 Lung Neoplasms Antibodies  >33 publications with PD-L1 and Lung Neoplasms

Organs/Tissues associated with anti-PD-L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >725 publications with PD-L1 and Blood
 Lymph Node Antibodies  >74 publications with PD-L1 and Lymph Node
 Lung Antibodies  >62 publications with PD-L1 and Lung
 Bone Antibodies  >55 publications with PD-L1 and Bone
 Skin Antibodies  >44 publications with PD-L1 and Skin
 Brain Antibodies  >31 publications with PD-L1 and Brain
 Pancreas Antibodies  >26 publications with PD-L1 and Pancreas
 Placenta Antibodies  >17 publications with PD-L1 and Placenta
 Stomach Antibodies  >12 publications with PD-L1 and Stomach
 Mouth Antibodies  >9 publications with PD-L1 and Mouth
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