EGFR ELISA Kit

Intended Uses: The human TNF-alpha ELISA kit is to be used for the in vitro quantitative determination of human TNF-alpha in serum, plasma, and cell culture supernatant samples. This kit is intended for laboratory research use only. The analysis should be performed by trained laboratory professionals!!Background/Introduction: Tumor necrosis factor alpha (TNF-alpha), a homotrimeric 17 kD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the p55 (RI) and p75 (RII) TNF-receptors. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.

Small volumes of EGFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495414 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the EGFR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EGFR. The ELISA analytical biochemical technique of the MBS495414 kit is based on EGFR antibody-EGFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).