Estriol ELISA Kit

Intended Uses: The Estradiol sensitive ELISA is an enzyme immunoassay for the quantitative measurement of Estradiol in serum and plasma

Principle of the Assay: The Estradiol sensitive ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Estradiol molecule. Endogenous Estradiol of a patient sample competes with an Estradiol horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverse proportional to the concentration of Estradiol in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Estradiol in the patient sample.

Small volumes of Estriol elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495282 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Estriol, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Estriol. The ELISA analytical biochemical technique of the MBS495282 kit is based on Estriol antibody-Estriol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Estriol antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Estriol. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).