F-E3 ELISA Kit for General

Description: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of F-E3 in serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with F-E3. During the reaction, Universal F-E3 in the sample or standard competes with a fixed amount of F-E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal F-E3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Universal F-E3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of F-E3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2501141 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the F-E3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F-E3. The ELISA analytical biochemical technique of the MBS2501141 kit is based on F-E3 antibody-F-E3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F-E3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F-E3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).