Hazelnut ELISA Kit

Principle of the Assay: The Hazelnut quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against hazelnut proteins is bound on the surface of a microtiter plate. Hazelnut containing samples or standards are given in-to the wells of the microtiter plate. After 20 minutes incu-bation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against hazelnut proteins is given into the wells and after 20 minutes of incubation the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of hazelnut is directly proportional to the colour intensity of the test sample.

Small volumes of Hazelnut elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495714 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hazelnut, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hazelnut. The ELISA analytical biochemical technique of the MBS495714 kit is based on Hazelnut antibody-Hazelnut antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hazelnut antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hazelnut. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).