Endomorphin-2 (EM-2) ELISA Kit for Rat

Name: Rat EM-2 ELISA Kit; Endomorphin-2; MyBioSource
Brand: MyBioSource
SKU: MBS043652
Price: 670 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS043652

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated EM-2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EM-2 antibody to coat Microelisa Stripplate wells… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

Endomorphin-2 (EM-2), ELISA Kit

Full Product Name

Rat Endomorphin-2 ELISA Kit

Product Gene Name

EM-2 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

6.25 pg/ml – 200 pg/ml.

Sensitivity

1.0 pg/ml.

Specificity

No significant cross-reactivity or interference between Rat EM-2 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

21,639 Da

3D Structure

ModBase 3D Structure for P27882

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated EM-2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat EM-2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EM-2 and EM-2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EM-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of EM-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EM-2 purchase

MBS043652 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Endomorphin-2 (EM-2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EM-2. The ELISA analytical biochemical technique of the MBS043652 kit is based on EM-2 antibody-EM-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EM-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EM-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EM-2. It may not necessarily be applicable to this product.

NCBI GI #

404218

NCBI Accession #

CAA48192.1

UniProt Primary Accession #

P27882

UniProt Secondary Accession #

D6VUG4

NCBI Official Full Name

ERV1

UniProt Protein Name

Mitochondrial FAD-linked sulfhydryl oxidase ERV1

UniProt Synonym Protein Names

14 kDa regulatory protein; Essential for respiration and vegetative growth protein 1

UniProt Gene Name

ERV1

UniProt Entry Name

ERV1_YEAST

UniProt Comments for EM-2

Function: FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins. Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13

Catalytic activity: 2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactor: FAD.

Subunit structure: Homodimer. Interacts with MIA40, forming transient intermolecular disulfide bridges. Ref.8 Ref.11 Ref.12

Subcellular location: Mitochondrion intermembrane space Ref.9 Ref.10.

Sequence similarities: Contains 1 ERV/ALR sulfhydryl oxidase domain.

Sequence caution: The sequence AAB48659.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA43129.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA48192.1 differs from that shown. Reason: Erroneous gene model prediction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EM-2 elisa kit

Reference Product	PubMed Publications
MIA40 elisa kit	>55 publications with EM-2 and MIA40
MRS11 elisa kit	>5 publications with EM-2 and MRS11
TIM13 elisa kit	>4 publications with EM-2 and TIM13
TIM9 elisa kit	>4 publications with EM-2 and TIM9
TIM8 elisa kit	>2 publications with EM-2 and TIM8
MIC17 elisa kit	>1 publications with EM-2 and MIC17
MIC14 elisa kit	>1 publications with EM-2 and MIC14
HOT13 elisa kit	>1 publications with EM-2 and HOT13
TIM22 elisa kit	>1 publications with EM-2 and TIM22