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FCGR2A Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8113782

This Human CD32A overexpression lysate was created in CHO Cells and intented for use as a Western blot (WB) positive control. Purification of CD32A protein from the overexpression lysate was verified. More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD32A (FCGR2A), Cell Lysate
Full Product Name
Human CD32A CHO Overexpression Lysate
Product Gene Name
FCGR2A cell lysate
Product Synonym Names
Human CD32a/FCGR2A CHO Cell Lysate (WB positive control); Fc fragment of IgG receptor IIa; Human CD32 Overexpression Lysate; Human CD32A Overexpression Lysate; Human CDw32 Overexpression Lysate; Human Fc gamma RIIA Overexpression Lysate; Human FCG2 Overexpression Lysate; Human FcGR Overexpression Lysate; Human FCGR2 Overexpression Lysate; Human FCGR2A1 Overexpression Lysate; Human IGFR2 Overexpression Lysate
Sequence
Met1-Ile21 8
Host
CHO Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
34,930 Da
Sequence Positions
196aa
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding (Met 1-Ile 218) of human CD32a (AAA35827.1) was expressed with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Product Categories/Family for FCGR2A cell lysate

Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules
Applications Tested/Suitable for FCGR2A cell lysate
Western Blot (WB)
Application Notes for FCGR2A cell lysate
WB: Use at an assay dependent dilution.

Other Notes

Small volumes of FCGR2A cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCGR2A purchase

MBS8113782 is a cell lysate targeting FCGR2A. Also known as Human CD32A CHO Overexpression Lysate. Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR2A. It may not necessarily be applicable to this product.

NCBI GI #
182474
NCBI GeneID
2212
NCBI Accession #
AAA35827.1
UniProt Secondary Accession #
Q8WUN1; Q8WW64
UniProt Related Accession #
P12318
NCBI Official Full Name
IgG Fc fragment receptor
NCBI Official Synonym Full Names
Fc fragment of IgG receptor IIa
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
Protein Family
Low affinity immunoglobulin gamma Fc region receptor
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a

NCBI Summary for FCGR2A

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
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Research Articles on FCGR2A

1. this study shows that FCGR genetic polymorphism affects antibody responses to GARP in patients with breast cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with FCGR2A cell lysate

 Products by Pathway  Pathway Diagram
 FCGR Activation Pathway antibodies  FCGR Activation Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Leishmaniasis Pathway antibodies  Leishmaniasis Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
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