GPA33 Cell Lysate – Tissue Lysate

Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

This Human A33 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of A33 protein from the overexpression lysate was verified.

Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74. Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6. Frey, D. et al., 2008, Cancer Biother Radiopharm 23 (1):65-73. Rageul, J. et al., 2009, Int J Cancer 125 (12):2802-9.

Small volumes of GPA33 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS8114505 is a cell lysate targeting GPA33. Also known as Human A33 HEK293 Overexpression Lysate. Host: HEK293 Cells.