Mouse Anti-CD133 Antibody – Cat# MBS666976

Binding of anti-CD133/APC to human Y-79 retinoblastoma

CD133 Antibody – Mouse

Name: CD133 Antibody; Mouse; Human-Reactive; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS666976
Price: 605 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS666976

Human CD133 is a 117kd 5 transmembrane protein expressed by a subset of hematopoetic stem cells found in blood and some other tissues. Increased expression of CD133 may be a predictor of decreased prognosis in patients with metastatic cancer(3). Antibody… More details

$605 / 120 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD133, Antibody

Full Product Name

anti-human CD133-APC

Product Gene Name

anti-CD133 antibody

Product Synonym Names

Monoclonal anti-human CD133(AC133)/APC; anti-human CD133; anti-CD133 Allophycocyanin; AC133; Prominin; PROML1; PROM1

Isotype

Mouse IgG1,k

Clone Number

ANC9C5

Host

Mouse

Species Reactivity

Human

Form/Format

50mM Sodium Phosphate pH7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%; BSA, 0.04% NaN3 (as a preservative).

Specificity

Antibody ANC9C5 recognizes epitope 1 of human CD133(AC133) present on full length CD133 transfectants, Y-79 retinoblastoma, and other cell types.

Preparation and Storage

Store at 2-5 degree C. Do not freeze! Protect from light.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

Conjugation

APC

Immunogen

WERI-Rb-1 human retinoblastoma cells

Production

Protein A purified antibody from tissue culture supernatant was conjugated to cross-linked Allophycocyanin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at approximately 0. 5 mg/ml with an A650/A280 ratio of 2.0.

Performance

Antibody ANC9C5 was tested for its ability to bind to full length CD133 transfectants Y-79 retinoblastoma cells, and other CD133+ cells i flow cytometry. Five x 10 5 human Y-79 tumor cells per tube were pre incubated with 20 ul of 250 ug/ml human Ig (to block non specific binding) after which they were incubate 45 minutes on ice with 80 ul of anti-CD133/APC at a dilution factor of 1:50 (10 ug/ml), after which they were washed three times, fixed and analyzed b Cells stained positive with a mean shift of 1.11 lo 10 fluorescent units when compared to a Mouse IgG1/APC negative control . Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml unconjugated anti-CD133 antibody .

Product Categories/Family for anti-CD133 antibody

Anti-Human Antibodies; Progenitor/Stem Cell

Applications Tested/Suitable for anti-CD133 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-CD133 antibody

Antibody ANC9C5 has been used to image cancer stem cells in a colon carcinoma xenograft (4).

Product References and Citations for anti-CD133 antibody

1) Miraglia S, Buck DW, et al. (1997) Blood 90(12): 5013-5021.; 2) Shmelkov SV, Rafii S, et al. (2005) Int J Biochem Cell Biol 37(4): 715-9.; 3) Mehra N, Voest EE, et al. (2006) Clin Canc Res 12(16): 4859-66.; 4) Jin ZH, Fuibayashi Y, et al. (2012) Mol Imaging 11: 445-50. PMID: 23084245.

Other Notes

Small volumes of anti-CD133 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD133 purchase

MBS666976 is an antibody targeting CD133. Also known as anti-human CD133-APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD133. It may not necessarily be applicable to this product.

Protein Family

Prominin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.