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CD2 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS489113

The original antibody was generated by immunizing mice with human T lymphocytes that had been activated with phorbol myristic acid (PMA).Describes the characterization of epitopes on the human T cell surface protein Tp50 using two antibodies.This chimeric… More details

$635 / 0.2 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD2, Antibody
Full Product Name
Anti-CD2 [35.1]
Product Gene Name
anti-CD2 antibody
Product Synonym Names
LFA-2; LFA-3 receptor; SRBC; Rosette receptor; T-cell surface antigen CD2; Erythrocyte receptor; T-cell surface antigen T11/Leu-5
Isotype
Rabbit IgG kappa
Clone Number
35.1
Species Reactivity
Human
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Purified. Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
This antibody recognizes an epitope on the extracellular domain of the human T cell surface protein Tp50 (CD2) between amino acid residues 39-50. The binding of this antibody does not inhibit the interaction between CD2 and CD58. CD2 is a glycoprotein belonging to the immunoglobulin superfamily. It acts as a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
39,448 Da
OMIM
186990
3D Structure
ModBase 3D Structure for P06729
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.

Product Categories/Family for anti-CD2 antibody

T cells; cell adhesion; cell signaling
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CD2 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-CD2 antibody
This antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity. This antibody was used in the generation of CD2 targeting ricin A chain-containing immunotoxins (PMID: 2461993). This antibody is capable of immunoprecipitating CD2 antigen form cell lysates (PMID: 8557753). This antibody was also used for the cell cycle analysis of cells along with other antibodies like anti-CD3, anti-CD4, and anti-CD8 using flow cytometry (PMID: 9881968).

Flow Cytometry (FC/FACS) of anti-CD2 antibody

Flow cytometry using anti-CD2 antibody 35.1 . Jurkat cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 35.1 (MBS489113, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
Anti-CD2 Antibody – Cat# MBS489113

Western Blot (WB) of anti-CD2 antibody

Western blot using anti-CD2 antibody 35.1 . Jurkat (A) and Molt-4 (B) cells lysate, and human thymus (C) and tonsil (D) tissue lysates (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 35.1 (MBS489113) at 0.01µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used, and protein was detected by chemiluminescence.
Anti-CD2 Antibody, Western Blot (WB) – Cat# MBS489113

Immunofluorescence (IF) of anti-CD2 antibody

Immunofluorescence staining of Jurkat cells with anti-CD2 antibody 35.1 . Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 35.1 (MBS489113) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, MBS489113, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Anti-CD2 Antibody, Immunofluorescence (IF) – Cat# MBS489113

Product References and Citations for anti-CD2 antibody

Martin et al.
J Immunol. 1983 Jul;131(1):180-5.

Other Notes

Small volumes of anti-CD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD2 purchase

MBS489113 is an antibody targeting CD2. Also known as Anti-CD2 [35.1]. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for CD2. It may not necessarily be applicable to this product.

NCBI GI #
156071472
NCBI GeneID
914
NCBI Accession #
NP_001758.2
UniProt Primary Accession #
P06729
UniProt Secondary Accession #
Q96TE5
UniProt Related Accession #
P06729
GenBank Nucleotide #
NM_001767.3
NCBI Official Full Name
T-cell surface antigen CD2
NCBI Official Synonym Full Names
CD2 molecule
NCBI Official Symbol
CD2
NCBI Official Synonym Symbols
T11; SRBC; LFA-2
UniProt Protein Name
T-cell surface antigen CD2
UniProt Synonym Protein Names
Erythrocyte receptor; LFA-2; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD_antigen: CD2
Protein Family
T-cell surface antigen
UniProt Gene Name
CD2
UniProt Synonym Gene Names
SRBC
UniProt Entry Name
CD2_HUMAN
NCBI Protein Information
T-cell surface antigen CD2; CD2 antigen (p50), sheep red blood cell receptor; LFA-3 receptor; T-cell surface antigen T11/Leu-5; erythrocyte receptor; lymphocyte-function antigen-2; rosette receptor

NCBI Summary for CD2

CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]
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UniProt Comments for CD2

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Cell surface; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: internal side of plasma membrane; cell surface; anchored to plasma membrane; integral to plasma membrane; plasma membrane; extracellular region; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell surface receptor linked signal transduction; T cell activation; apoptosis; natural killer cell activation; regulation of T cell differentiation; blood coagulation; lipid raft polarization; positive regulation of tumor necrosis factor production; leukocyte migration; positive regulation of myeloid dendritic cell activation
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Research Articles on CD2

1. Although sMCs displayed immunoreactivity for one of the neoplastic antigens in the majority of SM patients, the aberrant CD2 and/or CD25 expression on sMCs is not as indicative of SM as the BMMC immunophenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD2 antibody

 Reference Product  PubMed Publications
 CD58 antibody  >416 publications with CD2 and CD58
 PTPRC antibody  >344 publications with CD2 and PTPRC
 CD2AP antibody  >164 publications with CD2 and CD2AP
 CD48 antibody  >147 publications with CD2 and CD48
 LCK antibody  >79 publications with CD2 and LCK
 SH3KBP1 antibody  >19 publications with CD2 and SH3KBP1
 CD2BP2 antibody  >13 publications with CD2 and CD2BP2
 ITK antibody  >11 publications with CD2 and ITK
 CD8A antibody  >3 publications with CD2 and CD8A

Pathways associated with anti-CD2 antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-CD2 antibody

 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >872 publications with CD2 and Lymphoma
 Skin Diseases Antibodies  >328 publications with CD2 and Skin Diseases
 Necrosis Antibodies  >317 publications with CD2 and Necrosis
 Inflammation Antibodies  >241 publications with CD2 and Inflammation
 Kidney Diseases Antibodies  >154 publications with CD2 and Kidney Diseases
 Liver Diseases Antibodies  >127 publications with CD2 and Liver Diseases
 Skin Neoplasms Antibodies  >126 publications with CD2 and Skin Neoplasms
 Lung Diseases Antibodies  >105 publications with CD2 and Lung Diseases
 Vascular Diseases Antibodies  >103 publications with CD2 and Vascular Diseases
 Fever Antibodies  >101 publications with CD2 and Fever

Organs/Tissues associated with anti-CD2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4682 publications with CD2 and Blood
 Bone Antibodies  >773 publications with CD2 and Bone
 Muscle Antibodies  >543 publications with CD2 and Muscle
 Kidney Antibodies  >496 publications with CD2 and Kidney
 Thymus Antibodies  >438 publications with CD2 and Thymus
 Lymph Node Antibodies  >391 publications with CD2 and Lymph Node
 Heart Antibodies  >309 publications with CD2 and Heart
 Spleen Antibodies  >280 publications with CD2 and Spleen
 Intestine Antibodies  >254 publications with CD2 and Intestine
 Lung Antibodies  >187 publications with CD2 and Lung
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