Cd49d/VLA-4 Antibody – Mouse

IgG1

7.2

Mouse

Human

Purified (UNLB) Antibody

Human CD49d/VLA-4 CD49d, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha4 chain. CD49d associates noncovalently with CD29, the integrin beta1 subunit, to form the alpha4beta1 very late antigen-4 (VLA-4) heterodimer. Integrin alpha4 can also associate with integrin beta7 to form the alpha4beta7 heterodimer. It is expressed on most leukocytes, with the possible exception of neutrophils. VLA-4 mediates leukocyte binding to CD106/VCAM-1, and is involved in the migration of leukocytes from blood to tissue at sites of inflammation. VLA-4 can also provide a costimulatory signal to T cells for activation and proliferation. The alpha4beta7 integrin heterodimer is expressed on a small subpopulation of thymocytes, most lymph node T and B lymphocytes, NK cells and eosinophils. Integrin alpha4beta7 also binds to fibronectin and VCAM-1.1-4

The fluorescein (FITC), Alexa Fluor 488 (AF488), Alexa Fluor 647 (AF647), and Cyanine 5 (CY5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.

To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.

Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.