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CD49e Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1802469

CD49e (VLA5 alpha) is a type I transmembrane glycoprotein of the integrin alpha subclass (intergrin 5 alpha), expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells. It binds to RGD… More details

$385 / 100 Tests +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD49e, Antibody
Full Product Name
Anti-Hu CD49e PE
Product Gene Name
anti-CD49e antibody
Product Synonym Gene Name
ITGA5
Product Synonym Names
integrin subunit alpha 5; VLA5 alpha, integrin 5 alpha, FNRA
Isotype
Mouse IgG2b
Clone Number
SAM1
Host
Mouse
Species Reactivity
Non-human primates, Human
Form/Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Molecular Weight
114,536 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (54789045..54813050, complement). Location: 12q11-q13
OMIM
135620
3D Structure
ModBase 3D Structure for P08648
Immunogen
U937 cells
Conjugation
PE
Applications Tested/Suitable for anti-CD49e antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes for anti-CD49e antibody
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Flow Cytometry (FCM/FACS) of anti-CD49e antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49e (SAM1) PE antibody (10ul reagent/100ul of peripheral whole blood).
Mouse Anti-CD49e Antibody – Cat# MBS1802469

Flow Cytometry (FCM/FACS) of anti-CD49e antibody

Separation of human CD49e positive monocytes (red-filled) from CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49e (SAM1) PE antibody (10ul reagent/100ul of peripheral whole blood).
Mouse Anti-CD49e Antibody, Flow Cytometry (FCM/FACS) – Cat# MBS1802469

Product References and Citations for anti-CD49e antibody

*Blase L, Merling A, Engelmann S, Moller P, Schwartz-Albiez R: Characterization of cell surface-expressed proteochondroitin sulfate of pre-B Nalm-6 cells and its possible role in laminin adhesion. Leukemia 1996 Jun;10(6):1000-11., URL: https://pubmed.ncbi.nlm.nih.gov/8667635/,*Tachimoto H, Hudson SA, Bochner BS: Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation. J Allergy Clin Immunol. 2001 Feb;107(2):302-9, URL: https://pubmed.ncbi.nlm.nih.gov/11174197/,*Malara A, Gruppi C, Rebuzzini P, Visai L, Perotti C, Moratti R, Balduini C, Tira ME, Balduini A: Megakaryocyte-matrix interaction within bone marrow: new roles for fibronectin and factor XIII-A. Blood 2011 Feb 24;117(8):2476-83. , URL: https://pubmed.ncbi.nlm.nih.gov/21131589/,*Lessey BA, Castelbaum AJ, Buck CA, Lei Y, Yowell CW, Sun J: Further characterization of endometrial integrins during the menstrual cycle and in pregnancy. Fertil Steril. 1994 Sep;62(3):497-506., URL: https://pubmed.ncbi.nlm.nih.gov/8062944/,

Other Notes

Small volumes of anti-CD49e antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD49e purchase

MBS1802469 is an antibody targeting CD49e. Also known as Anti-Hu CD49e PE. Reactive in Non-human primates, Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD49e. It may not necessarily be applicable to this product.

NCBI GI #
56237029
NCBI GeneID
3678
NCBI Accession #
NP_002196.2
UniProt Primary Accession #
P08648
UniProt Secondary Accession #
Q96HA5
UniProt Related Accession #
P08648
GenBank Nucleotide #
NM_002205.2
NCBI Official Full Name
integrin alpha-5
NCBI Official Synonym Full Names
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
NCBI Official Symbol
ITGA5
NCBI Official Synonym Symbols
FNRA; CD49e; VLA5A
UniProt Protein Name
Integrin alpha-5
UniProt Synonym Protein Names
CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5
Protein Family
Integrin
UniProt Gene Name
ITGA5
UniProt Synonym Gene Names
FNRA
UniProt Entry Name
ITA5_HUMAN
NCBI Protein Information
integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit

NCBI Summary for CD49e

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD49e

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12q11-q13

Cellular Component: ruffle; focal adhesion; cell surface; cytoplasm; plasma membrane; synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; vascular endothelial growth factor receptor 2 binding; metal ion binding; platelet-derived growth factor receptor binding; epidermal growth factor receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; extracellular matrix organization and biogenesis; viral reproduction; cell-substrate adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; memory; heterophilic cell adhesion; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; angiogenesis; cell adhesion; blood coagulation; leukocyte migration; wound healing, spreading of epidermal cells; positive regulation of cell migration
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Research Articles on CD49e

1. Mechanical regulation of alpha5beta1 integrin-ligand interactions in a molecular mechanism for cell adhesion strengthening by cyclic forces.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD49e antibody

 Reference Product  PubMed Publications
 ITGB1 antibody  >7 publications with CD49e and ITGB1
 ITGB3 antibody  >6 publications with CD49e and ITGB3
 FN1 antibody  >6 publications with CD49e and FN1
 SPP1 antibody  >3 publications with CD49e and SPP1
 ITGB5 antibody  >3 publications with CD49e and ITGB5
 EGFR antibody  >2 publications with CD49e and EGFR
 COL1A1 antibody  >2 publications with CD49e and COL1A1
 COL18A1 antibody  >1 publications with CD49e and COL18A1

Pathways associated with anti-CD49e antibody

 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram

Diseases associated with anti-CD49e antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with CD49e and Neoplasms
 Neoplasm Invasiveness Antibodies  >7 publications with CD49e and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >6 publications with CD49e and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >6 publications with CD49e and Breast Neoplasms
 Carcinoma Antibodies  >5 publications with CD49e and Carcinoma
 Inflammation Antibodies  >3 publications with CD49e and Inflammation
 Cardiovascular Diseases Antibodies  >2 publications with CD49e and Cardiovascular Diseases
 Lung Diseases Antibodies  >2 publications with CD49e and Lung Diseases
 Nervous System Diseases Antibodies  >2 publications with CD49e and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with CD49e and Liver Diseases

Organs/Tissues associated with anti-CD49e antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >9 publications with CD49e and Vascular
 Bone Antibodies  >9 publications with CD49e and Bone
 Uterus Antibodies  >7 publications with CD49e and Uterus
 Embryonic Tissue Antibodies  >6 publications with CD49e and Embryonic Tissue
 Liver Antibodies  >5 publications with CD49e and Liver
 Bone Marrow Antibodies  >5 publications with CD49e and Bone Marrow
 Muscle Antibodies  >5 publications with CD49e and Muscle
 Lung Antibodies  >4 publications with CD49e and Lung
 Blood Antibodies  >3 publications with CD49e and Blood
 Connective Tissue Antibodies  >3 publications with CD49e and Connective Tissue
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