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CD66c Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1802265

CD66c is a GPI-anchored glycoprotein capable of homophilic adhesion and heterophilic binding to CD66a-e, CD62E, and galectins. It is expressed on granulocytes and epithelial cells, and has potential applications in the detection of sites of infection and… More details

$385 / 100 Tests +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD66c, Antibody
Full Product Name
Anti-Hu CD66c PE
Product Gene Name
anti-CD66c antibody
Product Synonym Gene Name
CEACAM6
Product Synonym Names
CEAL; NCA; CEACAM6; CEA cell adhesion molecule 6; CD66c
Isotype
Mouse IgG1 kappa
Clone Number
B6.2
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
Purity/Purification
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody B6.2 recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
Molecular Weight
37,195 Da
OMIM
M29541 mRNA
3D Structure
ModBase 3D Structure for P40199
Conjugation
PE
Immunogen
Extracts from human breast carcinoma cells
Excitation Laser
blue (488 nm)
Workshop
HLDA V: MA135, HLDA VI: MA86
Applications Tested/Suitable for anti-CD66c antibody
Flow Cytometry (FC/FACS)(QC tested)
Application Notes for anti-CD66c antibody
FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (1ml) is sufficient for 100 tests.

Flow Cytometry (FC/FACS) of anti-CD66c antibody

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD66c (B6.2) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD66c Antibody – Cat# MBS1802265

Flow Cytometry (FC/FACS) of anti-CD66c antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD66c (B6.2) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD66c Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1802265

Product References and Citations for anti-CD66c antibody

Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J: Patterns of reactivity of four novel monoclonal antibodies (B72.3, DF3, B1.1 and B6.2) with cells in human malignant and benign effusions. Acta Cytol. 1984 Jul-Aug;28(4):356-67., URL: https://www.ncbi.nlm.nih.gov/pubmed/6205527
Yamagami S, Yokoo S, Amano S, Ebihara N: Characterization of bone marrow derived cells in the substantia propria of the human conjunctiva. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4476-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/17898268
Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA: Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site. Blood. 2001 Sep 1;98(5):1469-79., URL: https://www.ncbi.nlm.nih.gov/pubmed/11520797
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K: Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow. J Biol Chem. 2008 Jun 6;283(23):15647-55., URL: https://pubmed.ncbi.nlm.nih.gov/18375392/

Other Notes

Small volumes of anti-CD66c antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD66c purchase

MBS1802265 is an antibody targeting CD66c. Also known as Anti-Hu CD66c PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD66c. It may not necessarily be applicable to this product.

NCBI GI #
612407822
NCBI GeneID
4680
NCBI Accession #
NP_002474.4
UniProt Primary Accession #
P40199
UniProt Secondary Accession #
Q13774; Q14920; Q53XP7
UniProt Related Accession #
P40199
GenBank Nucleotide #
NM_002483.6
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 6 preproprotein
NCBI Official Synonym Full Names
carcinoembryonic antigen related cell adhesion molecule 6
NCBI Official Symbol
CEACAM6
NCBI Official Synonym Symbols
NCA; CEAL; CD66c
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 6
UniProt Synonym Protein Names
Non-specific crossreacting antigen; Normal cross-reacting antigen; CD_antigen: CD66c
Protein Family
Carcinoembryonic antigen-related cell adhesion molecule
UniProt Gene Name
CEACAM6
UniProt Synonym Gene Names
NCA
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 6

NCBI Summary for CD66c

This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19. [provided by RefSeq, Apr 2014]
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UniProt Comments for CD66c

CEACAM6: a glycosylphosphatidylinositol(GPI)-linked glycoprotein implicated in a variety of human cancers. Expressed on epithelial cells of various tissues, especially colon, lung, pancreas, trachea, and bone marrow. Participates in innate immune defense, cell proliferation and differentiation. Expressed at high levels in gastrointestinal tract, pancreatic and lung tumors. CEACAM6 promotes tumor angiogenesis in gastric cancer. May enhance invasiveness and metastatic potential in gastric and pancreatic cancer by promoting EMT via PI3K/AKT signaling. Upregulated by the pro-inflammatory cytokine IL-6. Belongs to the immunoglobulin superfamily/CEA family.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell signaling; leukocyte migration; positive regulation of cell migration; positive regulation of cell proliferation; signal transduction
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Research Articles on CD66c

1. show the upregulation of the tumor-associated antigens CEACAM5/6 by trans-signaling of the pro-inflammatory cytokine IL-6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD66c antibody

 Products by Pathway  Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Fibronectin Matrix Formation Pathway antibodies  Fibronectin Matrix Formation Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with anti-CD66c antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >22 publications with CD66c and Inflammation
 Leukemia Antibodies  >17 publications with CD66c and Leukemia
 Lung Diseases Antibodies  >17 publications with CD66c and Lung Diseases
 Lung Neoplasms Antibodies  >16 publications with CD66c and Lung Neoplasms
 Breast Neoplasms Antibodies  >15 publications with CD66c and Breast Neoplasms
 Lymphoma Antibodies  >12 publications with CD66c and Lymphoma
 Necrosis Antibodies  >8 publications with CD66c and Necrosis
 Disease Models, Animal Antibodies  >7 publications with CD66c and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >6 publications with CD66c and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >5 publications with CD66c and Liver Neoplasms

Organs/Tissues associated with anti-CD66c antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >40 publications with CD66c and Blood
 Intestine Antibodies  >35 publications with CD66c and Intestine
 Lung Antibodies  >30 publications with CD66c and Lung
 Stomach Antibodies  >15 publications with CD66c and Stomach
 Bone Marrow Antibodies  >5 publications with CD66c and Bone Marrow
 Pancreas Antibodies  >5 publications with CD66c and Pancreas
 Bladder Antibodies  >4 publications with CD66c and Bladder
 Prostate Antibodies  >3 publications with CD66c and Prostate
 Skin Antibodies  >3 publications with CD66c and Skin
 Trachea Antibodies  >1 publications with CD66c and Trachea
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