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CHKB Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS853227

MBS853227 is an antibody targeting CHKB. Also known as CHKB Antibody. Reactive in Human. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHKB, Antibody
Full Product Name
CHKB Antibody
Product Gene Name
Product Synonym Names
Choline/ethanolamine kinase; Choline kinase beta (EC 2.7.1.32)(CK); Ethanolamine kinase (EC 2.7.1.82)(EK); CHKB; CHETK; CHKL
Species Reactivity
Human
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
CHKB antibody detects endogenous levels of total CHKB protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
13,506 Da
OMIM
612395
3D Structure
ModBase 3D Structure for Q9Y259
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human CHKB.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CHKB antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CHKB antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-CHKB antibody

Western blot analysis of extracts from HepG2 cells and A549 cells, using CHKB antibody.
Anti-CHKB Antibody – Cat# MBS853227

Product References and Citations for anti-CHKB antibody

Yamazaki N., J. Biol. Chem. 275:31739-31746(2000). Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004). Yu L.-R., J. Proteome Res. 6:4150-4162(2007).

Other Notes

Small volumes of anti-CHKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHKB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
choline/ethanolamine kinase
NCBI Official Synonym Full Names
choline kinase beta
NCBI Official Symbol
CHKB
NCBI Official Synonym Symbols
CK; EK; CKB; EKB; CHKL; CHETK; CKEKB; MDCMC
UniProt Protein Name
Choline/ethanolamine kinase
UniProt Synonym Protein Names
Choline kinase beta (EC:2.7.1.32); CK; CKB; Choline kinase-like protein; Ethanolamine kinase (EC:2.7.1.82); EK; Ethanolamine kinase beta; EKB; choline/ethanolamine kinase beta; CKEKB
UniProt Gene Name
CHKB
UniProt Synonym Gene Names
CHETK; CHKL; CK; CKB; EK; EKB; CKEKB
NCBI Protein Information
choline/ethanolamine kinase

NCBI Summary for CHKB

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]
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UniProt Comments for CHKB

Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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