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TIM-3 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS489215

The original antibody was generated by immunizing mice with recombinant TIM-3 protein.This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and… More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TIM-3, Antibody
Full Product Name
Anti-TIM-3 [7H2]
Product Gene Name
anti-TIM-3 antibody
Product Synonym Names
CD366; Hepatitis A virus cellular receptor 2; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; D5D5R; E3; CGMCCNO 20763
Isotype
Rabbit IgG kappa
Clone Number
7H2
Species Reactivity
Human
Form/Format
PBS with 0.02% Proclin 300.
Purity/Purification
Purified. Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
The original antibody is specific for TIM-3. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
301
OMIM
606652
3D Structure
ModBase 3D Structure for Q8TDQ0
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.

Product Categories/Family for anti-TIM-3 antibody

Tumor
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TIM-3 antibody
Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TIM-3 antibody
The antibody specifically detected TIM-3 protein in Western blot analysis. The antibody 7H2 and the commercial antibody D5D5R were used to stain tonsils and appendix tissue. Results showed the antibody specificity is comparable to the commercial antibody (CN113234158A).

Immunofluorescence (IF) of anti-TIM-3 antibody

Immunofluorescence staining of Daudi cells with anti-TIM-3 antibody 7H2 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 7H2 (MBS489215) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, MBS489215, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 7H2 (MBS489215, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.
Anti-TIM-3 Antibody – Cat# MBS489215

Other Notes

Small volumes of anti-TIM-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIM-3 purchase

MBS489215 is an antibody targeting TIM-3. Also known as Anti-TIM-3 [7H2]. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for TIM-3. It may not necessarily be applicable to this product.

NCBI GI #
311033536
NCBI GeneID
84868
NCBI Accession #
Q8TDQ0.3
UniProt Primary Accession #
Q8TDQ0
UniProt Secondary Accession #
Q8WW60; Q96K94; B2RAY2
UniProt Related Accession #
Q8TDQ0
NCBI Official Full Name
Hepatitis A virus cellular receptor 2
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
HAVCR2
NCBI Official Synonym Symbols
TIM3; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
UniProt Protein Name
Hepatitis A virus cellular receptor 2
UniProt Synonym Protein Names
T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3
Protein Family
Hepatitis A virus cellular receptor
UniProt Gene Name
HAVCR2
UniProt Synonym Gene Names
TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3
UniProt Entry Name
HAVR2_HUMAN
NCBI Protein Information
hepatitis A virus cellular receptor 2; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin mucin receptor 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglo

NCBI Summary for TIM-3

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
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UniProt Comments for TIM-3

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Disease: Rheumatoid Arthritis
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Research Articles on TIM-3

1. TIM-3 polymorphism is associated with increased susceptibility to Ankylosing spondylitis possibly by downregulating gene expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TIM-3 antibody

 Reference Product  PubMed Publications
 LGALS9 antibody  >46 publications with TIM-3 and LGALS9

Diseases associated with anti-TIM-3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >52 publications with TIM-3 and Inflammation
 Disease Models, Animal Antibodies  >41 publications with TIM-3 and Disease Models, Animal
 Lung Diseases Antibodies  >31 publications with TIM-3 and Lung Diseases
 Liver Diseases Antibodies  >30 publications with TIM-3 and Liver Diseases
 Necrosis Antibodies  >26 publications with TIM-3 and Necrosis
 Respiratory Hypersensitivity Antibodies  >21 publications with TIM-3 and Respiratory Hypersensitivity
 Neoplasms, Experimental Antibodies  >20 publications with TIM-3 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >15 publications with TIM-3 and Cardiovascular Diseases
 Skin Diseases Antibodies  >14 publications with TIM-3 and Skin Diseases
 Adenocarcinoma Antibodies  >13 publications with TIM-3 and Adenocarcinoma

Organs/Tissues associated with anti-TIM-3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >245 publications with TIM-3 and Blood
 Liver Antibodies  >25 publications with TIM-3 and Liver
 Spleen Antibodies  >20 publications with TIM-3 and Spleen
 Lung Antibodies  >13 publications with TIM-3 and Lung
 Kidney Antibodies  >11 publications with TIM-3 and Kidney
 Bone Marrow Antibodies  >11 publications with TIM-3 and Bone Marrow
 Heart Antibodies  >9 publications with TIM-3 and Heart
 Brain Antibodies  >7 publications with TIM-3 and Brain
 Intestine Antibodies  >3 publications with TIM-3 and Intestine
 Placenta Antibodies  >2 publications with TIM-3 and Placenta
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