TRIM5 / RNF88 Immunizing Blocking Peptide

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; ligase activity; pattern recognition receptor activity; protein binding; protein binding, bridging; protein homodimerization activity; protein kinase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; autophagy; defense response to virus; innate immune response; negative regulation of virion penetration into host cell; pattern recognition receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; positive regulation of transcription factor activity; regulation of lipopolysaccharide-mediated signaling pathway; regulation of protein localization; viral reproduction