Asprosin (FBN1) ELISA Kit for Human

Name: Human FBN1 ELISA Kit; Asprosin; MyBioSource
Brand: MyBioSource
SKU: MBS2709813
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2709813

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to asprosin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to asprosin. Next, Avidin… More details

$305 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Asprosin (FBN1), ELISA Kit

Full Product Name

Human Asprosin ELISA Kit

Product Gene Name

FBN1 elisa kit

Product Synonym Names

Asprosin/Asprosin

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.055ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of asprosin. No significant cross-reactivity or interference between asprosin and analogues was observed.

Preparation and Storage

Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level asprosin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level asprosin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

312,237 Da

Chromosome Location

Chromosome: 15; NC_000015.9 (48700503..48937985, complement). Location: 15q21.1

OMIM

102370

3D Structure

ModBase 3D Structure for P35555

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Product Categories/Family for FBN1 elisa kit

Endocrinology; Hormone metabolism

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Application Notes for FBN1 elisa kit

Enzyme-linked immunosorbent assay for Antigen Detection.

Other Notes

Small volumes of FBN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FBN1 purchase

MBS2709813 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Asprosin (FBN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FBN1. The ELISA analytical biochemical technique of the MBS2709813 kit is based on FBN1 antibody-FBN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FBN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FBN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FBN1. It may not necessarily be applicable to this product.

NCBI GI #

281485550

NCBI GeneID

2200

NCBI Accession #

NP_000129.3

UniProt Primary Accession #

P35555

UniProt Secondary Accession #

Q15972; Q75N87; B2RUU0; D2JYH6

UniProt Related Accession #

P35555

GenBank Nucleotide #

NM_000138.4

NCBI Official Full Name

fibrillin-1

NCBI Official Synonym Full Names

fibrillin 1

NCBI Official Symbol

FBN1

NCBI Official Synonym Symbols

FBN; SGS; WMS; MASS; MFS1; OCTD; SSKS; WMS2; ACMICD; ECTOL1; GPHYSD2

UniProt Protein Name

Fibrillin-1

Protein Family

Fibrillin

UniProt Gene Name

FBN1

UniProt Synonym Gene Names

FBN

UniProt Entry Name

FBN1_HUMAN

NCBI Protein Information

fibrillin-1; fibrillin 15

NCBI Summary for FBN1

This gene encodes a member of the fibrillin family. The encoded protein is a large, extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq, Jul 2008]

UniProt Comments for FBN1

FBN1: a large, extracellular matrix glycoprotein of the fibrillin family that serves as a structural component of 10-12 nm calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1- containing microfibrils provide long-term force bearing structural support in elastic and nonelastic connective tissue throughout the body. May regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. Defects in this protein are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. Interacts with COL16A1.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; microfibril; extracellular region; basement membrane

Molecular Function: protein binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; metanephros development; skeletal development

Disease: Ectopia Lentis 1, Isolated, Autosomal Dominant; Mass Syndrome; Weill-marchesani Syndrome 2; Stiff Skin Syndrome; Acromicric Dysplasia; Marfan Syndrome; Geleophysic Dysplasia 2

Research Articles on FBN1

1. Report immunolocalization of fibrillin-1/perlecan in human fetal intervertebral disc.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FBN1 elisa kit

Reference Product	PubMed Publications
FBN2 elisa kit	>31 publications with FBN1 and FBN2
LTBP1 elisa kit	>3 publications with FBN1 and LTBP1
DCN elisa kit	>3 publications with FBN1 and DCN
ELN elisa kit	>2 publications with FBN1 and ELN
FBLN2 elisa kit	>2 publications with FBN1 and FBLN2
VCAN elisa kit	>1 publications with FBN1 and VCAN
MYOC elisa kit	>1 publications with FBN1 and MYOC
MFAP5 elisa kit	>1 publications with FBN1 and MFAP5
MFAP2 elisa kit	>1 publications with FBN1 and MFAP2
CALR elisa kit	>1 publications with FBN1 and CALR

Pathways associated with FBN1 elisa kit

Products by Pathway	Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with FBN1 elisa kit

Disease Name	Pubmed Publications
Marfan Syndrome Antibodies	>403 publications with FBN1 and Marfan Syndrome
Cardiovascular Diseases Antibodies	>363 publications with FBN1 and Cardiovascular Diseases
Heart Diseases Antibodies	>346 publications with FBN1 and Heart Diseases
Ectopia Lentis Antibodies	>76 publications with FBN1 and Ectopia Lentis
Aortic Aneurysm, Thoracic Antibodies	>46 publications with FBN1 and Aortic Aneurysm, Thoracic
Arachnodactyly Antibodies	>31 publications with FBN1 and Arachnodactyly
Aortic Rupture Antibodies	>18 publications with FBN1 and Aortic Rupture
Neoplasms Antibodies	>13 publications with FBN1 and Neoplasms
Weill-Marchesani Syndrome Antibodies	>11 publications with FBN1 and Weill-Marchesani Syndrome
ECTOPIA LENTIS 1, ISOLATED, AUTOSOMAL DOMINANT Antibodies	>10 publications with FBN1 and ECTOPIA LENTIS 1, ISOLATED, AUTOSOMAL DOMINANT

Organs/Tissues associated with FBN1 elisa kit

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>187 publications with FBN1 and Connective Tissue
Vascular Antibodies	>73 publications with FBN1 and Vascular
Heart Antibodies	>52 publications with FBN1 and Heart
Skin Antibodies	>52 publications with FBN1 and Skin
Muscle Antibodies	>36 publications with FBN1 and Muscle
Eye Antibodies	>34 publications with FBN1 and Eye
Bone Antibodies	>32 publications with FBN1 and Bone
Lung Antibodies	>16 publications with FBN1 and Lung
Embryonic Tissue Antibodies	>12 publications with FBN1 and Embryonic Tissue
Kidney Antibodies	>9 publications with FBN1 and Kidney