CD200 ELISA Kit for Human

Human

Colorimetric sandwich

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

31.2pg/ml-2,000pg/ml

<10pg/ml

Natural and recombinant Human CD200

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

32,975 Da

AY603771 mRNA

ModBase 3D Structure for P41217

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.015|0.062|0.099|0.185|0.329|0.625|1.179|2.046

Sample|1|2|3|
n|16|16|16|
Mean(pg/ml)|89|252|897|
Standard deviation|4.36|16.12|69.06|
CV(%)|4.9%|6.4%|7.7%|

Sample|1|2|3|
n|24|24|24
Mean(pg/ml)|75|251|960
Standard deviation|4.12|14.30|72
CV(%)|5.5%|5.7%|7.5%

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 89|75|93|98|88|4.66|5.3%
Sample 2: 252|245|216|254|241|7.47|3.1%
Sample 3: 897|862|897|871|881|22.02|2.5%
*number of samples for each test n=16.

There is no detectable cross-reactivity with other relevant proteins.

Expression system for standard: NS0; Immunogen sequence: Q31-G232

heparin or EDTA

The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human CD200 with <10pg/ml sensitivity. Format: 96-well plate with removable strips.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.

Human CD200 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD200 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD200. The detection antibody is a HRP linked goat polyclonal antibody processed by a proprietary method. The kit contains recombinant Human CD200 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q31-G232. The kit is analytically validated with ready to use reagents.

To measure Human CD200, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CD200 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD200 in the sample.

2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml

15

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.