CHD3 ELISA Kit for Human

Name: Human CHD3 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS2504837
Price: 300 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2504837

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CHD3. Standards or samples are added to the micro ELISA plate wells and combined with… More details

$300 / 24 Tests (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

CHD3, ELISA Kit

Full Product Name

Human CHD3 (Chromodomain Helicase DNA Binding Protein 3) ELISA Kit

Product Gene Name

CHD3 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.31-20ng/mL

Sensitivity

0.19ng/mL

Specificity

This kit recognizes Human CHD3 in samples. No significant cross-reactivity or interference between Human CHD3 and analogues was observed.

Preparation and Storage

Store at 4 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CHD3 were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CHD3 were tested 20 times on one plate, respectively.

Molecular Weight

233,037 Da

OMIM

602120

3D Structure

ModBase 3D Structure for Q12873

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CHD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHD3 purchase

MBS2504837 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CHD3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHD3. The ELISA analytical biochemical technique of the MBS2504837 kit is based on CHD3 antibody-CHD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CHD3. It may not necessarily be applicable to this product.

NCBI GI #

158420731

NCBI GeneID

1107

NCBI Accession #

NP_001005271.2

UniProt Primary Accession #

Q12873

UniProt Secondary Accession #

Q9Y4I0; D3DTQ9; E9PG89

UniProt Related Accession #

Q12873

GenBank Nucleotide #

NM_001005271.2

NCBI Official Full Name

chromodomain-helicase-DNA-binding protein 3 isoform 3

NCBI Official Synonym Full Names

chromodomain helicase DNA binding protein 3

NCBI Official Symbol

CHD3

NCBI Official Synonym Symbols

ZFH; Mi-2a; Mi2-ALPHA

UniProt Protein Name

Chromodomain-helicase-DNA-binding protein 3

UniProt Synonym Protein Names

ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH

Protein Family

Chromodomain-helicase-DNA-binding protein

UniProt Gene Name

CHD3

UniProt Synonym Gene Names

CHD-3; hZFH

UniProt Entry Name

CHD3_HUMAN

NCBI Protein Information

chromodomain-helicase-DNA-binding protein 3; ATP-dependent helicase CHD3; CHD-3; hZFH; mi-2 autoantigen 240 kDa protein; zinc finger helicase; zinc-finger helicase (Snf2-like)

NCBI Summary for CHD3

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

UniProt Comments for CHD3

CHD-3 iso3: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity. Widely expressed. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; centrosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; helicase activity; ATP binding

Biological Process: chromatin assembly or disassembly; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; centrosome organization and biogenesis; chromatin modification; spindle organization and biogenesis; DNA duplex unwinding

Research Articles on CHD3

1. In this study, the chromatin remodeler chromodomain helicase DNA binding (CHD3) protein is identified as an important component of the initial repression of the herpesvirus genome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CHD3 elisa kit

Reference Product	PubMed Publications
CHD4 elisa kit	>13 publications with CHD3 and CHD4
SUMO1 elisa kit	>5 publications with CHD3 and SUMO1
HDAC2 elisa kit	>4 publications with CHD3 and HDAC2
HDAC1 elisa kit	>4 publications with CHD3 and HDAC1
MTA1 elisa kit	>3 publications with CHD3 and MTA1
MBD2 elisa kit	>3 publications with CHD3 and MBD2
MTA2 elisa kit	>2 publications with CHD3 and MTA2
RBBP4 elisa kit	>1 publications with CHD3 and RBBP4

Pathways associated with CHD3 elisa kit

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HDACs Deacetylate Histones Pathway antibodies	HDACs Deacetylate Histones Pathway Diagram
RNA Polymerase I Promoter Clearance Pathway antibodies	RNA Polymerase I Promoter Clearance Pathway Diagram
RNA Polymerase I Transcription Pathway antibodies	RNA Polymerase I Transcription Pathway Diagram
RNA Polymerase I Transcription Initiation Pathway antibodies	RNA Polymerase I Transcription Initiation Pathway Diagram
RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies	RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
Signaling Events Mediated By HDAC Class I Pathway antibodies	Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with CHD3 elisa kit

Disease Name	Pubmed Publications
Ataxia Antibodies	>3 publications with CHD3 and Ataxia
Nervous System Diseases Antibodies	>3 publications with CHD3 and Nervous System Diseases
Heart Diseases Antibodies	>2 publications with CHD3 and Heart Diseases
Neoplasm Metastasis Antibodies	>1 publications with CHD3 and Neoplasm Metastasis
Lymphoma Antibodies	>1 publications with CHD3 and Lymphoma
Death Antibodies	>1 publications with CHD3 and Death
Movement Disorders Antibodies	>1 publications with CHD3 and Movement Disorders
Fibrosis Antibodies	>1 publications with CHD3 and Fibrosis
Inflammation Antibodies	>1 publications with CHD3 and Inflammation

Organs/Tissues associated with CHD3 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with CHD3 and Kidney
Brain Antibodies	>4 publications with CHD3 and Brain
Nerve Antibodies	>3 publications with CHD3 and Nerve
Embryonic Tissue Antibodies	>2 publications with CHD3 and Embryonic Tissue
Bone Antibodies	>2 publications with CHD3 and Bone
Muscle Antibodies	>2 publications with CHD3 and Muscle
Testis Antibodies	>1 publications with CHD3 and Testis
Pancreas Antibodies	>1 publications with CHD3 and Pancreas
Mouth Antibodies	>1 publications with CHD3 and Mouth
Intestine Antibodies	>1 publications with CHD3 and Intestine