Complement C3a Des Arg ELISA Kit for Human

Name: Human ELISA Kit; Complement C3a Des Arg; MyBioSource
Brand: MyBioSource
SKU: MBS494467
Price: 1080 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS494467

Competitive immunoassay (ELISA) for the determination of C3a des Arg in plasma. For research use only. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

Complement C3a des Arg, ELISA Kit

Full Product Name

Complement C3a des Arg (Human) ELISA

Species Reactivity

Human

Assay Type

Competitive

Samples

Human C3a des Arg plasma in EDTA/Futhan tubes

Detection Range

0 / 0.313 - 20.0 ng/mL

Sensitivity

0.120 ng/mL

Preparation and Storage

All components of this kit, except the conjugate and lyophilized standard, are stable at 4 degree C until the kit's expiration date.The human C3a des Arg conjugate and lyophilized standard should be stored at -20 degree C upon receipt.

Standards

7 calibrators, serially diluted from 1 prepared lyophilized calibrator.

Sample Volume

100 uL of properly prepared/diluted unknown / determination.

Design

Enzyme immunoassay (ELISA) technique.

Description Protocol

The Complement C3a des Arg (human), EIA kit is a competitive immunoassay for the determination of C3a des Arg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C3a des Arg to bind, in a competitive manner, the human C3a des Arg in the sample or an alkaline phosphatase molecule which has human C3a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C3a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of human C3a des Arg. For further explanation of the principles and practice of immunoassays please see the excellent books by Chard1 or Tijssen2.

Assay Description

1 hour incubation (RT) + 2 hours (RT) + 1 hour (37 degree C) = 4 hours total incubation time.

Product Categories/Family for Complement C3a des Arg elisa kit

Immunology; Research Assays; Assay Categories

Other Notes

Small volumes of Complement C3a des Arg elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Complement C3a des Arg purchase

MBS494467 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement C3a des Arg, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Complement C3a des Arg. The ELISA analytical biochemical technique of the MBS494467 kit is based on Complement C3a des Arg antibody-Complement C3a des Arg antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Complement C3a des Arg antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Complement C3a des Arg. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).