The TFact ETV4 DNA-Binding ELISA detects active ETV4 in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. Hela cells were stimulated by PMA (200nM) before harvest.

ETV4 ELISA Kit for Human

Name: Human ETV4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS9502162
Price: 595 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9502162

MBS9502162 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ETV4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive… More details

$595 / 1 Kit +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

ETV4, ELISA Kit

Full Product Name

ETV4 TFact DNA-Binding ELISA Kit

Product Gene Name

ETV4 elisa kit

Product Synonym Names

ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; ETV4; E1AF

Species Reactivity

Human

Assay Type

DNA-Binding ELISA

Samples

Cell lysate

Sensitivity

10-30 ug of total protein/well

Form/Format

12 x 8-Well Microstrips

Specificity

The TFact ETV4 DNA-Binding ELISA Kit detects endogenous levels of total ETV4 protein.

Preparation and Storage

Store entire kit at 4 degree C until use.

Molecular Weight

23,422 Da

OMIM

U18018 mRNA

3D Structure

ModBase 3D Structure for P43268

Detection Method

Colorimetric 450 nm

Subtype

None

Other Notes

Small volumes of ETV4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ETV4. It may not necessarily be applicable to this product.

NCBI GI #

118918421

NCBI GeneID

2118

NCBI Accession #

NP_001073143.1

UniProt Primary Accession #

P43268

UniProt Secondary Accession #

Q96AW9; A8K314; B7Z5J3; B7Z9J6

UniProt Related Accession #

P43268

GenBank Nucleotide #

NM_001079675.2

NCBI Official Full Name

ETS translocation variant 4 isoform 1

NCBI Official Synonym Full Names

ETS variant 4

NCBI Official Symbol

ETV4

NCBI Official Synonym Symbols

E1AF; PEA3; E1A-F; PEAS3

UniProt Protein Name

ETS translocation variant 4

UniProt Synonym Protein Names

Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog; Protein PEA3

Protein Family

ETS translocation

UniProt Gene Name

ETV4

UniProt Synonym Gene Names

E1AF; PEA3; Protein PEA3

NCBI Protein Information

ETS translocation variant 4

UniProt Comments for ETV4

ETV4: Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. Belongs to the ETS family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: cell differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Research Articles on ETV4

1. four oncogenic ETS (ERG, ETV1, ETV4, and ETV5), and no other ETS, interact with the Ewing's sarcoma breakpoint protein, EWS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ETV4 elisa kit

Products by Pathway	Pathway Diagram
LKB1 Signaling Events Pathway antibodies	LKB1 Signaling Events Pathway Diagram
Prostate Cancer Pathway antibodies	Prostate Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with ETV4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>89 publications with ETV4 and Neoplasms
Prostatic Neoplasms Antibodies	>33 publications with ETV4 and Prostatic Neoplasms
Carcinoma Antibodies	>29 publications with ETV4 and Carcinoma
Neoplasm Metastasis Antibodies	>18 publications with ETV4 and Neoplasm Metastasis
Gastrointestinal Diseases Antibodies	>11 publications with ETV4 and Gastrointestinal Diseases
Breast Neoplasms Antibodies	>10 publications with ETV4 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>6 publications with ETV4 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>5 publications with ETV4 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>5 publications with ETV4 and Lung Neoplasms
Lung Diseases Antibodies	>4 publications with ETV4 and Lung Diseases

Organs/Tissues associated with ETV4 elisa kit

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>38 publications with ETV4 and Prostate
Kidney Antibodies	>14 publications with ETV4 and Kidney
Lung Antibodies	>7 publications with ETV4 and Lung
Bone Antibodies	>6 publications with ETV4 and Bone
Mouth Antibodies	>4 publications with ETV4 and Mouth
Embryonic Tissue Antibodies	>4 publications with ETV4 and Embryonic Tissue
Testis Antibodies	>3 publications with ETV4 and Testis
Stomach Antibodies	>3 publications with ETV4 and Stomach
Blood Antibodies	>3 publications with ETV4 and Blood
Mammary Gland Antibodies	>2 publications with ETV4 and Mammary Gland