FLRT2 ELISA Kit for Human

Principle of the Assay: The Picokine Human FGFBP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FGFBP1 with a 96-well strip plate that is pre-coated with antibody specific for FGFBP1. The detection antibody is a biotinylated antibody specific for FGFBP1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human FGFBP1 with immunogen: Expression system for standard: NSO, Immunogen sequence: K24-C234. The kit is analytically validated with ready to use reagents. To measure Human FGFBP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human FGFBP1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FGFBP1 in the sample!!Background/Introduction: Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4.

Small volumes of FLRT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1751713 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FLRT2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLRT2. The ELISA analytical biochemical technique of the MBS1751713 kit is based on FLRT2 antibody-FLRT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLRT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLRT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).