• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Gelsolin (GS) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2701701
SPECIAL PROMOTIONAL PRICING for a limited time

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gelsolin (GS). Standards or samples are then added to the appropriate microtiter plate wells with… More details

$275 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Gelsolin (GS), ELISA Kit
Full Product Name
Human Gelsolin (GS) ELISA Kit
Product Gene Name
GS elisa kit
Product Synonym Names
GSN; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
Species Reactivity
Human
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
62.5-4000pg/mL
Sensitivity
< 28.2pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Gelsolin (GS).
No significant cross-reactivity or interference between Gelsolin (GS) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
80,929 Da
3D Structure
ModBase 3D Structure for Q68FP1
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Gelsolin (GS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Gelsolin (GS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of GS elisa kit

Human GS ELISA Kit – Cat# MBS2701701

Other Notes

Small volumes of GS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GS purchase

MBS2701701 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gelsolin (GS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GS. The ELISA analytical biochemical technique of the MBS2701701 kit is based on GS antibody-GS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GS. It may not necessarily be applicable to this product.

NCBI GI #
51854227
NCBI GeneID
296654
NCBI Accession #
NP_001004080.1
UniProt Primary Accession #
Q68FP1
UniProt Related Accession #
Q68FP1
GenBank Nucleotide #
NM_001004080.1
NCBI Official Full Name
gelsolin
NCBI Official Synonym Full Names
gelsolin
NCBI Official Symbol
Gsn
UniProt Protein Name
Gelsolin
UniProt Synonym Protein Names
Actin-depolymerizing factor; ADF; Brevin
UniProt Gene Name
Gsn
UniProt Synonym Gene Names
ADF
NCBI Protein Information
gelsolin

NCBI Summary for GS

plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

UniProt Comments for GS

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p11

Cellular Component: actin cap; actin cytoskeleton; cortical actin cytoskeleton; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; lamellipodium; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; podosome; protein complex; ruffle; sarcoplasm

Molecular Function: actin binding; actin filament binding; calcium ion binding; myosin II binding; protein domain specific binding

Biological Process: actin filament capping; actin filament polymerization; actin filament severing; actin nucleation; actin polymerization or depolymerization; aging; apoptosis; barbed-end actin filament capping; extracellular matrix disassembly; negative regulation of virion penetration into host cell; oligodendrocyte development; phagocytosis, engulfment; phosphoinositide-mediated signaling; positive regulation of actin nucleation; protein destabilization; regulation of cell adhesion; response to ethanol; response to folic acid; sequestering of actin monomers; striated muscle atrophy; tissue regeneration; vesicle-mediated transport; wound healing
Show more

Research Articles on GS

1. Myelin loss and subsequent axon degeneration contributes to a variety of neurological diseases, such as multiple sclerosis (MS).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GS elisa kit

 Reference Product  PubMed Publications
 Casp3 elisa kit  >6 publications with GS and Casp3
 Ttr elisa kit  >1 publications with GS and Ttr
 App elisa kit  >1 publications with GS and App

Pathways associated with GS elisa kit

 Products by Pathway  Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with GS elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >25 publications with GS and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >10 publications with GS and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >8 publications with GS and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >7 publications with GS and Carcinoma, Hepatocellular
 Inflammation Antibodies  >6 publications with GS and Inflammation
 Urinary Bladder Neoplasms Antibodies  >6 publications with GS and Urinary Bladder Neoplasms
 Liver Diseases Antibodies  >6 publications with GS and Liver Diseases
 Liver Neoplasms Antibodies  >6 publications with GS and Liver Neoplasms
 Heart Diseases Antibodies  >4 publications with GS and Heart Diseases
 Lung Diseases Antibodies  >4 publications with GS and Lung Diseases

Organs/Tissues associated with GS elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >35 publications with GS and Brain
 Nerve Antibodies  >28 publications with GS and Nerve
 Liver Antibodies  >11 publications with GS and Liver
 Heart Antibodies  >10 publications with GS and Heart
 Lung Antibodies  >7 publications with GS and Lung
 Kidney Antibodies  >5 publications with GS and Kidney
 Artery Antibodies  >5 publications with GS and Artery
 Embryonic Tissue Antibodies  >3 publications with GS and Embryonic Tissue
 Adipose Tissue Antibodies  >3 publications with GS and Adipose Tissue
 Testis Antibodies  >2 publications with GS and Testis
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000