Il-1beta ELISA Kit for Human

Principle of the Assay: IL-1beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. The Human IL-1beta Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-1beta with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1beta. It contains E. coli-expressed recombinant Human IL-1beta. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1beta. The kit is analytically validated with ready to use reagents.

Small volumes of IL-1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS697346 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-1beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1beta. The ELISA analytical biochemical technique of the MBS697346 kit is based on IL-1beta antibody-IL-1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).