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Interleukin 3 (IL3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2122166
SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 3 (IL3). Standards or samples are then added to the appropriate… More details

$445 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 3 (IL3), ELISA Kit
Full Product Name
Human Mini Samples Interleukin 3 (IL3) ELISA Kit
Product Gene Name
IL3 elisa kit
Product Synonym Names
MCGF; MULTI-CSF; MCSF; M-CSF; Multiple; Multilineage-Colony-Stimulating Factor; Hematopoietic Growth Factor; P-Cell Stimulating Factor; Mast-Cell Growth Factor
Species Reactivity
Human
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
5.7pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 3 (IL3).
No significant cross-reactivity or interference between Mini Samples Interleukin 3 (IL3) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
OMIM
147740
3D Structure
ModBase 3D Structure for P08700
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 3 (IL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 3 (IL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Product Categories/Family for IL3 elisa kit

Cytokine; Infection Immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of IL3 elisa kit

Human IL3 ELISA Kit – Cat# MBS2122166

Other Notes

Small volumes of IL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL3 purchase

MBS2122166 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 3 (IL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL3. The ELISA analytical biochemical technique of the MBS2122166 kit is based on IL3 antibody-IL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL3. It may not necessarily be applicable to this product.

NCBI GI #
3002475
NCBI GeneID
3562
NCBI Accession #
AAC08706.1
UniProt Primary Accession #
P08700
UniProt Related Accession #
P08700
NCBI Official Full Name
IL-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
UniProt Protein Name
Interleukin-3
UniProt Synonym Protein Names
Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
Protein Family
Interleukin
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
UniProt Entry Name
IL3_HUMAN
NCBI Protein Information
interleukin-3

NCBI Summary for IL3

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL3

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Cytokine; Oncoprotein; Apoptosis; Secreted; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; cell-cell signaling; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
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Research Articles on IL3

1. Stimulated with either GM-CSF, IL-3, or IL-5 for 48 h.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL3 elisa kit

 Reference Product  PubMed Publications
 IL2 elisa kit  >80 publications with IL3 and IL2
 IL5 elisa kit  >48 publications with IL3 and IL5
 JAK2 elisa kit  >13 publications with IL3 and JAK2
 CSF2RB elisa kit  >10 publications with IL3 and CSF2RB
 IL7 elisa kit  >8 publications with IL3 and IL7
 FOS elisa kit  >7 publications with IL3 and FOS
 IL3RA elisa kit  >7 publications with IL3 and IL3RA
 GRB2 elisa kit  >2 publications with IL3 and GRB2
 PIK3CA elisa kit  >1 publications with IL3 and PIK3CA

Pathways associated with IL3 elisa kit

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with IL3 elisa kit

 Disease Name  Pubmed Publications
 Hematologic Diseases Antibodies  >49 publications with IL3 and Hematologic Diseases
 Bone Marrow Diseases Antibodies  >37 publications with IL3 and Bone Marrow Diseases
 Necrosis Antibodies  >27 publications with IL3 and Necrosis
 Disease Models, Animal Antibodies  >25 publications with IL3 and Disease Models, Animal
 Lung Diseases Antibodies  >20 publications with IL3 and Lung Diseases
 Inflammation Antibodies  >17 publications with IL3 and Inflammation
 Drug Toxicity Antibodies  >17 publications with IL3 and Drug Toxicity
 Skin Diseases Antibodies  >16 publications with IL3 and Skin Diseases
 Asthma Antibodies  >14 publications with IL3 and Asthma
 Anemia Antibodies  >12 publications with IL3 and Anemia

Organs/Tissues associated with IL3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >11 publications with IL3 and Mixed
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