Meprin A Beta (MEP1b) ELISA Kit for Human

Name: Human MEP1b ELISA Kit; Meprin A Beta; MyBioSource
Brand: MyBioSource
SKU: MBS2701437
Price: 285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2701437

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Beta (MEP1b). Standards or samples are then added to the appropriate microtiter plate… More details

$285 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Meprin A Beta (MEP1b), ELISA Kit

Full Product Name

Human Meprin A Beta (MEP1b) ELISA Kit

Product Gene Name

MEP1b elisa kit

Product Synonym Names

PPH Beta; Endopeptidase-2; Meprin B; PABA Peptide Hydrolase; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Beta

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Tissue Homogenates, Cell Lysates and Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

< 0.117ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Meprin A Beta (MEP1b).
No significant cross-reactivity or interference between Meprin A Beta (MEP1b) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

79,571 Da

OMIM

X81333 mRNA

3D Structure

ModBase 3D Structure for Q16820

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Meprin A Beta (MEP1b) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Meprin A Beta (MEP1b) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Other Notes

Small volumes of MEP1b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MEP1b purchase

MBS2701437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Meprin A Beta (MEP1b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MEP1b. The ELISA analytical biochemical technique of the MBS2701437 kit is based on MEP1b antibody-MEP1b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MEP1b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MEP1b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MEP1b. It may not necessarily be applicable to this product.

NCBI GI #

153070264

NCBI GeneID

4225

NCBI Accession #

NP_005916.2

UniProt Primary Accession #

Q16820

UniProt Secondary Accession #

Q670J1; B7ZM35; B9EGL6

UniProt Related Accession #

Q16820

GenBank Nucleotide #

NM_005925.2

NCBI Official Full Name

meprin A subunit beta isoform 1

NCBI Official Synonym Full Names

meprin A subunit beta

NCBI Official Symbol

MEP1B

UniProt Protein Name

Meprin A subunit beta

UniProt Synonym Protein Names

Endopeptidase-2; Meprin B; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta

Protein Family

Meprin

UniProt Gene Name

MEP1B

NCBI Protein Information

meprin A subunit beta

NCBI Summary for MEP1b

Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]

UniProt Comments for MEP1b

Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.

Research Articles on MEP1b

1. TSPAN8 might be important for the orchestration of meprin beta at the cell surface with impact on certain proteolytic processes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MEP1b elisa kit

Reference Product	PubMed Publications
MEP1A elisa kit	>4 publications with MEP1b and MEP1A
NPY elisa kit	>1 publications with MEP1b and NPY
GRP elisa kit	>1 publications with MEP1b and GRP

Pathways associated with MEP1b elisa kit

Products by Pathway	Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram

Diseases associated with MEP1b elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>2 publications with MEP1b and Kidney Diseases
Neoplasms Antibodies	>1 publications with MEP1b and Neoplasms
Kidney Neoplasms Antibodies	>1 publications with MEP1b and Kidney Neoplasms

Organs/Tissues associated with MEP1b elisa kit

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>2 publications with MEP1b and Intestine
Muscle Antibodies	>1 publications with MEP1b and Muscle