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Norepinephrine Transporter (NET) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2021571
SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NET in human tissue homogenates, cell lysates and other biological fluids.Principle of the Assay: The microplate provided in this kit has been pre-coated with an… More details

$425 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Norepinephrine Transporter (NET), ELISA Kit
Full Product Name
Human Norepinephrine Transporter (NET) ELISA Kit
Product Gene Name
NET elisa kit
Product Synonym Names
SLC6A2; NAT1; NET; NET1; Sodium-dependent noradrenaline transporter; Noradrenaline Transporter; Solute Carrier Family 6 member 2; Neurotransmitter transporter; Noradrenalin
Species Reactivity
Human
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.51ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of NET. No significant cross-reactivity or interference between NET and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NET were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NET were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
57,370 Da
OMIM
163970
3D Structure
ModBase 3D Structure for P23975
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Product Categories/Family for NET elisa kit

Endocrinology
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of NET elisa kit

Human NET ELISA Kit – Cat# MBS2021571

Other Notes

Small volumes of NET elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NET purchase

MBS2021571 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Norepinephrine Transporter (NET) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NET. The ELISA analytical biochemical technique of the MBS2021571 kit is based on NET antibody-NET antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NET antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NET. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NET. It may not necessarily be applicable to this product.

NCBI GI #
1143479
NCBI GeneID
6530
NCBI Accession #
CAA62566.1
UniProt Primary Accession #
P23975
UniProt Secondary Accession #
Q96KH8; B2R707; B4DX48
UniProt Related Accession #
P23975
NCBI Official Full Name
norepinephrine transporter
NCBI Official Synonym Full Names
solute carrier family 6 (neurotransmitter transporter), member 2
NCBI Official Symbol
SLC6A2
NCBI Official Synonym Symbols
NET; NAT1; NET1; SLC6A5
UniProt Protein Name
Sodium-dependent noradrenaline transporter
UniProt Synonym Protein Names
Norepinephrine transporter; NET; Solute carrier family 6 member 2
Protein Family
Sodium-dependent noradrenaline transporter
UniProt Gene Name
SLC6A2
UniProt Synonym Gene Names
NAT1; NET1; SLC6A5; NET
UniProt Entry Name
SC6A2_HUMAN
NCBI Protein Information
sodium-dependent noradrenaline transporter

NCBI Summary for NET

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
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UniProt Comments for NET

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: neuron projection; cell surface; membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; monoamine transmembrane transporter activity; beta-tubulin binding; alpha-tubulin binding; norephinephrine:sodium symporter activity

Biological Process: response to drug; synaptic transmission; norepinephrine transport; transport; neurotransmitter transport; monoamine transport; response to pain; transmembrane transport

Disease: Orthostatic Intolerance
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Research Articles on NET

1. A Cox regression analysis for remission incidence during the 8-week treatment course significantly depends on SLC6A2 variants (rs28386840, rs40434, and rs187714)from major depression after venlafaxine treatment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NET elisa kit

 Reference Product  PubMed Publications
 SLC18A2 elisa kit  >13 publications with NET and SLC18A2
 PICK1 elisa kit  >2 publications with NET and PICK1
 STX1A elisa kit  >1 publications with NET and STX1A

Pathways associated with NET elisa kit

 Products by Pathway  Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Na+/Cl- Dependent Neurotransmitter Transporters Pathway antibodies  Na+/Cl- Dependent Neurotransmitter Transporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway antibodies  Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway Diagram

Diseases associated with NET elisa kit

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >124 publications with NET and Mental Disorders
 Attention Deficit Disorder with Hyperactivity Antibodies  >51 publications with NET and Attention Deficit Disorder with Hyperactivity
 Nervous System Diseases Antibodies  >50 publications with NET and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >48 publications with NET and Cardiovascular Diseases
 Depressive Disorder Antibodies  >38 publications with NET and Depressive Disorder
 Heart Diseases Antibodies  >32 publications with NET and Heart Diseases
 Brain Diseases Antibodies  >31 publications with NET and Brain Diseases
 Disease Models, Animal Antibodies  >26 publications with NET and Disease Models, Animal
 Hypertension Antibodies  >26 publications with NET and Hypertension
 Tachycardia Antibodies  >17 publications with NET and Tachycardia

Organs/Tissues associated with NET elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >279 publications with NET and Brain
 Nerve Antibodies  >267 publications with NET and Nerve
 Muscle Antibodies  >44 publications with NET and Muscle
 Kidney Antibodies  >28 publications with NET and Kidney
 Placenta Antibodies  >18 publications with NET and Placenta
 Intestine Antibodies  >2 publications with NET and Intestine
 Testis Antibodies  >2 publications with NET and Testis
 Prostate Antibodies  >1 publications with NET and Prostate
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