Prothymosin Alpha (PTMa) ELISA Kit for Human

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PTMa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PTMa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTMa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTMa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTMa in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Small volumes of PTMa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2019930 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prothymosin Alpha (PTMa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PTMa. The ELISA analytical biochemical technique of the MBS2019930 kit is based on PTMa antibody-PTMa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PTMa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PTMa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).