Talin (TLN) ELISA Kit for Human

Name: Human TLN ELISA Kit; Talin; MyBioSource
Brand: MyBioSource
SKU: MBS2021424
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2021424

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Talin (TLN). Standards or samples are then added to the appropriate microtiter plate wells with a… More details

$405 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Talin (TLN), ELISA Kit

Full Product Name

Human Talin (TLN) ELISA Kit

Product Gene Name

TLN elisa kit

Product Synonym Names

Talin Protein; TLN1; ILWEQ

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Tissue homogenates, Cell lysates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.055ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Talin (TLN).
No significant cross-reactivity or interference between Talin (TLN) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

269,767 Da

OMIM

186745

3D Structure

ModBase 3D Structure for Q9Y490

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Talin (TLN) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Talin (TLN) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for TLN elisa kit

Signal transduction; Developmental science

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of TLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TLN purchase

MBS2021424 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Talin (TLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TLN. The ELISA analytical biochemical technique of the MBS2021424 kit is based on TLN antibody-TLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TLN. It may not necessarily be applicable to this product.

NCBI GI #

6682361

NCBI GeneID

7094

NCBI Accession #

AAF23322.1

UniProt Primary Accession #

Q9Y490

UniProt Secondary Accession #

Q86YD0; Q9NZQ2; Q9UHH8; Q9UPX3; A6NMY0

UniProt Related Accession #

Q9Y490

NCBI Official Full Name

talin

NCBI Official Synonym Full Names

talin 1

NCBI Official Symbol

TLN1

NCBI Official Synonym Symbols

TLN; ILWEQ

UniProt Protein Name

Talin-1

UniProt Gene Name

TLN1

UniProt Synonym Gene Names

KIAA1027; TLN

UniProt Entry Name

TLN1_HUMAN

NCBI Protein Information

talin-1

NCBI Summary for TLN

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

UniProt Comments for TLN

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; extracellular region; plasma membrane; microtubule organizing center; intercellular junction; cytosol

Molecular Function: actin filament binding; integrin binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding

Biological Process: axon guidance; intercellular junction assembly; platelet activation; cytoskeletal anchoring; unfolded protein response; platelet degranulation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; blood coagulation; cell motility

Research Articles on TLN

1. Data (including data from molecular dynamic simulations) suggest specific interactions between glycoprotein GPIIb/GPIIIa complex transmembrane/C-terminal domains and talin-1 in cell membrane environment during platelet activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TLN elisa kit

Reference Product	PubMed Publications
ITGB1 elisa kit	>3 publications with TLN and ITGB1
PTK2 elisa kit	>3 publications with TLN and PTK2
ITGB5 elisa kit	>1 publications with TLN and ITGB5
VCL elisa kit	>1 publications with TLN and VCL
ITGA5 elisa kit	>1 publications with TLN and ITGA5
ITGB3 elisa kit	>1 publications with TLN and ITGB3

Pathways associated with TLN elisa kit

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
EGF Receptor (ErbB1) Signaling Pathway antibodies	EGF Receptor (ErbB1) Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway antibodies	GRB2:SOS Provides Linkage To MAPK Signaling For Integrins Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with TLN elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>4 publications with TLN and Cardiovascular Diseases
Liver Diseases Antibodies	>4 publications with TLN and Liver Diseases
Liver Neoplasms Antibodies	>4 publications with TLN and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>4 publications with TLN and Carcinoma, Hepatocellular
Breast Neoplasms Antibodies	>4 publications with TLN and Breast Neoplasms
Neoplasm Invasiveness Antibodies	>4 publications with TLN and Neoplasm Invasiveness
Death Antibodies	>2 publications with TLN and Death
Prostatic Neoplasms Antibodies	>2 publications with TLN and Prostatic Neoplasms
Prostatic Diseases Antibodies	>2 publications with TLN and Prostatic Diseases
Hypertrophy Antibodies	>1 publications with TLN and Hypertrophy

Organs/Tissues associated with TLN elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with TLN and Blood
Bone Antibodies	>5 publications with TLN and Bone
Vascular Antibodies	>5 publications with TLN and Vascular
Liver Antibodies	>4 publications with TLN and Liver
Muscle Antibodies	>3 publications with TLN and Muscle
Prostate Antibodies	>3 publications with TLN and Prostate
Thyroid Antibodies	>2 publications with TLN and Thyroid
Mouth Antibodies	>2 publications with TLN and Mouth
Placenta Antibodies	>2 publications with TLN and Placenta
Heart Antibodies	>2 publications with TLN and Heart