Thymidinephosphorylase (TP) ELISA Kit for Human

Name: Human TP ELISA Kit; Thymidinephosphorylase; MyBioSource
Brand: MyBioSource
SKU: MBS2804000
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2804000

MBS2804000 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymidinephosphorylase (TP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Thymidinephosphorylase (TP), ELISA Kit

Full Product Name

Human Thymidinephosphorylase (TP) ELISA Kit

Product Gene Name

TP elisa kit

Product Synonym Names

ECGF1; MNGIE; PDECGF; TP; hPD-ECGF; OTTHUMP00000196769|endothelial cell growth factor 1 (platelet-derived)|gliostatin

Species Reactivity

Human

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other biological fluids

Detection Range

1.56-100ng/mL

Sensitivity

0.78ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Human TYMP. No significant cross-reactivity or interference between Human TYMP and analogues was observed.

Preparation and Storage

Store at 2-8 degree C. Please refer to Instruction Manual.

Molecular Weight

49,955 Da

OMIM

131222

3D Structure

ModBase 3D Structure for P19971

Detection Wavelength

450nm

Assay Duration

1-4.5h

Sample Volume

1-200uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV

Test Principle

This assay employs a two-site sandwich ELISA to quantitate TYMP in samples. An antibody specific for TYMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TYMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TP. It may not necessarily be applicable to this product.

NCBI GI #

166158925

NCBI GeneID

1890

NCBI Accession #

NP_001107228.1

UniProt Primary Accession #

P19971

UniProt Secondary Accession #

Q13390; Q8WVB7; A8MW15; H9KVA0

UniProt Related Accession #

P19971

GenBank Nucleotide #

NM_001113756.2

NCBI Official Full Name

thymidine phosphorylase isoform 1 proprotein

NCBI Official Synonym Full Names

thymidine phosphorylase

NCBI Official Symbol

TYMP

NCBI Official Synonym Symbols

TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF

UniProt Protein Name

Thymidine phosphorylase

UniProt Synonym Protein Names

Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase

Protein Family

Trehalose phosphorylase

UniProt Gene Name

TYMP

UniProt Synonym Gene Names

ECGF1; TP; PD-ECGF

UniProt Entry Name

TYPH_HUMAN

NCBI Protein Information

thymidine phosphorylase; tdRPase; gliostatin

NCBI Summary for TP

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

UniProt Comments for TP

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Phosphorylase; EC 2.4.2.4; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Cytokine; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; DNA replication

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; chemotaxis; DNA replication; cell differentiation

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)

Research Articles on TP

1. We suggest that a chip including DPYD, TYMS, TYMP, TK1, and TK2 genes is a potential tool to predict response in LARC following fluoropyrimidine-based CCRT

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TP elisa kit

Reference Product	PubMed Publications
DPYD elisa kit	>6 publications with TP and DPYD
UPP1 elisa kit	>3 publications with TP and UPP1
TK1 elisa kit	>1 publications with TP and TK1
NT5C elisa kit	>1 publications with TP and NT5C

Pathways associated with TP elisa kit

Products by Pathway	Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Pyrimidine Catabolism Pathway antibodies	Pyrimidine Catabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram

Diseases associated with TP elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>28 publications with TP and Nervous System Diseases
Brain Diseases Antibodies	>26 publications with TP and Brain Diseases
Neoplasms Antibodies	>23 publications with TP and Neoplasms
Mitochondrial neurogastrointestinal encephalopathy syndrome Antibodies	>9 publications with TP and Mitochondrial neurogastrointestinal encephalopathy syndrome
Colorectal Neoplasms Antibodies	>9 publications with TP and Colorectal Neoplasms
Carcinoma Antibodies	>7 publications with TP and Carcinoma
Liver Diseases Antibodies	>7 publications with TP and Liver Diseases
Adenocarcinoma Antibodies	>5 publications with TP and Adenocarcinoma
Diarrhea Antibodies	>5 publications with TP and Diarrhea
Vomiting Antibodies	>4 publications with TP and Vomiting

Organs/Tissues associated with TP elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>20 publications with TP and Muscle
Blood Antibodies	>13 publications with TP and Blood
Brain Antibodies	>12 publications with TP and Brain
Liver Antibodies	>11 publications with TP and Liver
Skin Antibodies	>4 publications with TP and Skin
Nerve Antibodies	>4 publications with TP and Nerve
Intestine Antibodies	>3 publications with TP and Intestine
Lung Antibodies	>2 publications with TP and Lung
Heart Antibodies	>2 publications with TP and Heart
Bone Antibodies	>2 publications with TP and Bone