Il-4 ELISpot Kit for Human

Human. No cross reactivity with other human cytokines.

The assay recognizes natural human IL-4. To define specificity, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-10 IL-12, IFNgamma, IL-2, IL-6, TNFalpha, IL-8 and IL-13) This testing was performed using the equivalent human IL-4 antibody pair in an ELISA assay.

15,023 Da

ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.