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hsa-miR-4445-3p miRNA – Hsa-Mir-4445-3p (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8317199

Synthetic miRNA Antagomir is used to inhibit the activity of target hsa-miR-4445-3p mRNA. More details

$410 / 10 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
hsa-miR-4445-3p, miRNA
Full Product Name
hsa-miR-4445-3p miRNA Antagomir
Product Gene Name
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Specificity
Antagomir is chemically-modified single-strand miRNA inhibitor functioning by blocking miRNA regulation of target gene expression efficiently. They are synthesized to reduce the ability of endogenous miRNAs to silence target mRNA transcripts. They can down-regulate the corresponding endogenous miRNAs. Our miRNA antagomir is single-strand miRNA inhibitor carrying the chemically modifications functioning by blocking miRNA regulation of target gene expression efficiently.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year. Avoid freeze-thaw cycles after reconstitution.
Components
This synthetic miRNA is based on the mature miRNA sequence. The strand of the antagomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. Stability of miRNA antagomir appears to be significantly higher than miRNA inhibitors. It exhibits enhanced cellular uptake, stability and regulatory activity and is recommended for miRNA functional studies in vitro and in vivo.
Directions for Use
Briefly centrifuge tubes containing miRNA antagomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA antagomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 10 nmol miRNA antagomir to 20 uM using 500 ul DEPC H2O (or RNase-free water). Pipette the solution up and down 3-5 times (or vortex briefly). Briefly centrifuge tubes containing miRNA antagomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA antagomir into small volumes and store at <= -20 degree C. miRNA antagomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times.

Product Categories/Family for hsa-miR-4445-3p mirna

Other Notes

Small volumes of hsa-miR-4445-3p mirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hsa-miR-4445-3p purchase

MBS8317199 is an miRNA targeting hsa-miR-4445-3p. Also known as hsa-miR-4445-3p miRNA Antagomir. Reactive in Human. Host: Synthetic.

Additional Details

NCBI/Uniprot data below describe general gene information for hsa-miR-4445-3p. It may not necessarily be applicable to this product.

NCBI Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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