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hsa-miR-520b miRNA – Hsa-Mir-520b (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8310969

Synthetic miRNA Agomir is used to regulate the expression of target hsa-miR-520b mRNA. More details

$410 / 5 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
hsa-miR-520b, miRNA
Full Product Name
hsa-miR-520b miRNA Agomir
Product Gene Name
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Specificity
Agomir is chemically-modified double-strand miRNA mimics which can mimic mature endogenous miRNAs after transfection into cells. They can up-regulate the endogenous miRNA activity by utilizing the natural miRNA machinery. Our miRNA agomir is designed to mimic mature miRNAs, and chemically modified to increase their stability and activity.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year. Avoid freeze-thaw cycles after reconstitution.
Components
This synthetic miRNA is based on the mature miRNA sequence. The antisense strand of the agomir has 2 phosphorothioates at the 5' end, 4 phosphorothioates, 1 cholesterol group at the 3' end, and full-length nucleotide 2'-methoxy modification. It exhibits enhanced cellular uptake, stability and regulatory activity in vivo.
Directions for Use
Briefly centrifuge tubes containing miRNA agomir to ensure that the miRNA pellet is located at the bottom of the tube. Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: dissolve 5 nmol miRNA agomir to 20 uM using 250 ul DEPC H2O (or RNase-free water). Pipette the solution up and down 3-5 times (or vortex briefly). Briefly centrifuge tubes containing miRNA agomir to ensure that the solution is collected at the bottom of the tube. Aliquot the miRNA agomir into small volumes and store at <= -20 degree C. miRNA agomir is stable (for 6 months under the specified storage condition). For best results, use in 3 months and limit freeze-thaw events for each tube no more than five times.

Product Categories/Family for hsa-miR-520b mirna

Other Notes

Small volumes of hsa-miR-520b mirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hsa-miR-520b purchase

MBS8310969 is an miRNA targeting hsa-miR-520b. Also known as hsa-miR-520b miRNA Agomir. Reactive in Human. Host: Synthetic.

Additional Details

NCBI/Uniprot data below describe general gene information for hsa-miR-520b. It may not necessarily be applicable to this product.

NCBI Accession #

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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