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Histone H1 (H1) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859677

MBS859677 is an antibody targeting H1. Also known as Histone H1 (Ab-17) Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H1, Antibody
Full Product Name
Histone H1 (Ab-17) Antibody
Product Gene Name
Product Synonym Names
H1 histone family; member 5; H1.5; H15; H1F5; HIST1H1B; histone 1; histone H1.5; histone H1a
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
Histone H1 (Ab-17) antibody detects endogenous levels of total Histone H1 protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
22,580 Da
OMIM
142711
3D Structure
ModBase 3D Structure for P16401
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (E-K-TP-P-V).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-H1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-H1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Testing Data of anti-H1 antibody

Peptide-+
Anti-H1 Antibody – Cat# MBS859677

Immunohistochemistry (IHC) of anti-H1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H1 (Ab-17) antibody. Western blot analysis of extracts from HepG2 cells, using Histone H1 (Ab-17) antibody.
Anti-H1 Antibody, Immunohistochemistry (IHC) – Cat# MBS859677

Immunofluorescence (IF) of anti-H1 antibody

serum +- Immunofluorescence analysis of HuvEc cells, treated with serum (20%, 15mins), using Histone H1 (Ab-17) antibody.
Anti-H1 Antibody, Immunofluorescence (IF) – Cat# MBS859677

Product References and Citations for anti-H1 antibody

Albig W., Gene 184:141-148(1997). Marzluff W.F., Genomics 80:487-498(2002). Olsen J.V., Cell 127:635-648(2006).

Other Notes

Small volumes of anti-H1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H1.5
NCBI Official Synonym Full Names
histone cluster 1 H1 family member b
NCBI Official Symbol
HIST1H1B
NCBI Official Synonym Symbols
H1; H1B; H1.5; H1F5; H1s-3
UniProt Protein Name
Histone H1.5
UniProt Synonym Protein Names
Histone H1a; Histone H1b; Histone H1s-3
Protein Family
UniProt Gene Name
HIST1H1B
UniProt Synonym Gene Names
H1F5
NCBI Protein Information
histone H1.5

NCBI Summary for H1

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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UniProt Comments for H1

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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