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SEPARASE Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859119

MBS859119 is an antibody targeting SEPARASE. Also known as SEPARASE (Ab-801) Antibody. Reactive in Human, Mouse. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SEPARASE, Antibody
Full Product Name
SEPARASE (Ab-801) Antibody
Product Gene Name
Product Synonym Names
Caspase-like protein ESPL1; EC 3.4.22.49; ESP1; ESPL1; Extra spindle poles-like 1 protein; KIAA0165; Separin
Species Reactivity
Human, Mouse
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
SEPARASE (Ab-801) antibody detects endogenous levels of total SEPARASE protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
197,745 Da
OMIM
604143
3D Structure
ModBase 3D Structure for Q14674
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human SEPARASE around the phosphorylation site of serine 801(N-S-SP-P-V).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SEPARASE antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-SEPARASE antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000

Testing Data of anti-SEPARASE antibody

Peptide-+
Anti-SEPARASE Antibody – Cat# MBS859119

Immunohistochemistry (IHC) of anti-SEPARASE antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Ab-801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Ab-801) antibody.
Anti-SEPARASE Antibody, Immunohistochemistry (IHC) – Cat# MBS859119

Immunofluorescence (IF) of anti-SEPARASE antibody

Peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Ab-801) antibody.
Anti-SEPARASE Antibody, Immunofluorescence (IF) – Cat# MBS859119

Product References and Citations for anti-SEPARASE antibody

Waizenegger I., Curr. Biol. 12:1368-1378(2002). Nagase T., DNA Res. 3:17-24(1996). Zou H., Science 285:418-422(1999).

Other Notes

Small volumes of anti-SEPARASE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SEPARASE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
separin
NCBI Official Synonym Full Names
extra spindle pole bodies like 1, separase
NCBI Official Symbol
ESPL1
NCBI Official Synonym Symbols
ESP1; SEPA
UniProt Protein Name
Separin
UniProt Synonym Protein Names
Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
Protein Family
UniProt Gene Name
ESPL1
UniProt Synonym Gene Names
ESP1; KIAA0165
NCBI Protein Information
separin

NCBI Summary for SEPARASE

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
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UniProt Comments for SEPARASE

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SEPARASE antibody

 Reference Product  PubMed Publications
 PTTG1 antibody  >2 publications with SEPARASE and PTTG1
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