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4E-BP1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS857453

MBS857453 is an antibody targeting 4E-BP1. Also known as 4E-BP1 (Ab-45) Antibody. Reactive in Human, Mouse, Rat. More details

$235 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
4E-BP1, Antibody
Full Product Name
4E-BP1 (Ab-45) Antibody
Product Gene Name
anti-4E-BP1 antibody
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
4E-BP1 (Ab-45) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
12,580 Da
OMIM
602223
3D Structure
ModBase 3D Structure for Q13541
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

Product Categories/Family for anti-4E-BP1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-4E-BP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-4E-BP1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100

Immunohistochemistry (IHC) of anti-4E-BP1 antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody.
Anti-4E-BP1 Antibody – Cat# MBS857453

Western Blot (WB) of anti-4E-BP1 antibody

Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody and 4E-BP1 (phospho-Thr45) antibody.
Anti-4E-BP1 Antibody, Western Blot (WB) – Cat# MBS857453

Product References and Citations for anti-4E-BP1 antibody

Gingras AC, et al. (1998) Genes Dev 12(4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904. Kumar V, et al. (2000) EMBO J 19(5): 1087-1097. Moody CA, et al. (2005) J Virol 79(9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Other Notes

Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for 4E-BP1. It may not necessarily be applicable to this product.

NCBI GI #
4758258
NCBI GeneID
1978
NCBI Accession #
NP_004086.1
UniProt Primary Accession #
Q13541
UniProt Secondary Accession #
Q6IBN3; B2R502; D3DSW8
UniProt Related Accession #
Q13541
GenBank Nucleotide #
NM_004095.3
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Protein Family
Human immunodeficiency virus type I enhancer-binding protein
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1

NCBI Summary for 4E-BP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
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UniProt Comments for 4E-BP1

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
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Research Articles on 4E-BP1

1. These results demonstrate a previously unrecognized role of IL24 in inhibition of translation, mediated through both phosphorylation of eIF2alpha and dephosphorylation of 4E-BP1, and provide the first direct evidence for translation control of gene-specific expression by IL24

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-4E-BP1 antibody

 Reference Product  PubMed Publications
 EIF4E antibody  >460 publications with 4E-BP1 and EIF4E
 MTOR antibody  >444 publications with 4E-BP1 and MTOR
 AKT1 antibody  >49 publications with 4E-BP1 and AKT1
 MAPK1 antibody  >22 publications with 4E-BP1 and MAPK1
 ATM antibody  >11 publications with 4E-BP1 and ATM
 RPS6KB1 antibody  >3 publications with 4E-BP1 and RPS6KB1
 MAPK14 antibody  >2 publications with 4E-BP1 and MAPK14
 EIF4E2 antibody  >2 publications with 4E-BP1 and EIF4E2
 BUB1 antibody  >1 publications with 4E-BP1 and BUB1

Pathways associated with anti-4E-BP1 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram

Diseases associated with anti-4E-BP1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >241 publications with 4E-BP1 and Neoplasms
 Hypertrophy Antibodies  >40 publications with 4E-BP1 and Hypertrophy
 Breast Neoplasms Antibodies  >38 publications with 4E-BP1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >34 publications with 4E-BP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >31 publications with 4E-BP1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >20 publications with 4E-BP1 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >18 publications with 4E-BP1 and Drug Toxicity
 Kidney Diseases Antibodies  >16 publications with 4E-BP1 and Kidney Diseases
 Necrosis Antibodies  >16 publications with 4E-BP1 and Necrosis
 Leukemia, Myeloid, Acute Antibodies  >16 publications with 4E-BP1 and Leukemia, Myeloid, Acute

Organs/Tissues associated with anti-4E-BP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >161 publications with 4E-BP1 and Muscle
 Blood Antibodies  >110 publications with 4E-BP1 and Blood
 Liver Antibodies  >46 publications with 4E-BP1 and Liver
 Vascular Antibodies  >44 publications with 4E-BP1 and Vascular
 Heart Antibodies  >40 publications with 4E-BP1 and Heart
 Brain Antibodies  >40 publications with 4E-BP1 and Brain
 Kidney Antibodies  >34 publications with 4E-BP1 and Kidney
 Lung Antibodies  >33 publications with 4E-BP1 and Lung
 Pancreas Antibodies  >21 publications with 4E-BP1 and Pancreas
 Prostate Antibodies  >17 publications with 4E-BP1 and Prostate
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