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DEPDC5 Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8502990

MBS8502990 is an antibody targeting DEPDC5. Also known as DEPDC5 Antibody. Reactive in Reacts with: HumanPredicted: Rat, MouseNot yet tested in other species.. Host: Rabbit. More details

$430 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DEPDC5, Antibody
Full Product Name
DEPDC5 Antibody
Product Gene Name
Host
Rabbit
Species Reactivity
Reacts with: Human
Predicted: Rat, Mouse
Not yet tested in other species.
Form/Format
Rabbit anti-DEPDC5 antibody purified by protein A column containing 0.02% NaN3
Preparation and Storage
Already reconstituted in 200 ul sterile H2O.
The antibody is stable for one year when stored at -20°C after reconstitution.
Avoid repeated freeze/defrost cycles.
OMIM
604364
Host Note
Rabbit polyclonal DEPDC5 (1) antibody was raised against a recombinate human DEPDC5 protein 129aa-332aa (BC057797).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DEPDC5 antibody
ELISA, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-DEPDC5 antibody
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:200-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.

Western Blot (WB) of anti-DEPDC5 antibody

Rabbit Anti-DEPDC5 Antibody – Cat# MBS8502990

Immunohistochemistry (IHC) of anti-DEPDC5 antibody

Rabbit Anti-DEPDC5 Antibody, Immunohistochemistry (IHC) – Cat# MBS8502990

Other Notes

Small volumes of anti-DEPDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DEPDC5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens DEP domain containing 5, mRNA
NCBI Official Synonym Full Names
DEP domain containing 5
NCBI Official Symbol
DEPDC5
NCBI Official Synonym Symbols
DEP.5; FFEVF; FFEVF1
UniProt Protein Name
GATOR complex protein DEPDC5
UniProt Synonym Protein Names
DEP domain-containing protein 5
UniProt Gene Name
DEPDC5
NCBI Protein Information
GATOR complex protein DEPDC5

NCBI Summary for DEPDC5

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
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UniProt Comments for DEPDC5

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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