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eNOS Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8510123

MBS8510123 is an antibody targeting eNOS. Also known as eNOS (Ab-1176) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
eNOS, Antibody
Full Product Name
eNOS (Ab-1176) Antibody
Product Gene Name
Product Synonym Names
Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
eNOS (Ab-1176) antibody detects endogenous levels of eNOS protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
67,862 Da
OMIM
163729
3D Structure
ModBase 3D Structure for P29474
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176(T-Q-SP-F-S).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-eNOS antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-eNOS antibody
WB: 1:500~1:1000
ELISA: 1:40000

Western Blot (WB) of anti-eNOS antibody

Western blot analysis of extracts from Hela cells treated with Insulin (0.01U/ml, 15min), using eNOS (Ab-1176) antibody.
Anti-eNOS Antibody – Cat# MBS8510123

Product References and Citations for anti-eNOS antibody

Yong Chool Boo, Am J Physiol Heart Circ Physiol, Nov 2002; 283: 1819. Christian Pott, Am J Physiol Cell Physiol, May 2006; 290: C1437-C1445. Monica Montagnani, J. Biol. Chem., Aug 2001; 276: 30392-30398. Peter F. Mount, Am J Physiol Renal Physiol, Nov 2005; 289: F1103-F1115.

Other Notes

Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nitric oxide synthase, endothelial isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 3
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Protein Family
UniProt Gene Name
NOS3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
NCBI Protein Information
nitric oxide synthase, endothelial

NCBI Summary for eNOS

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
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UniProt Comments for eNOS

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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