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FOXO3A Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS857276

MBS857276 is an antibody targeting FOXO3A. Also known as FOXO3A (Ab-253) Antibody. Reactive in Human, Mouse, Rat. More details

$235 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FOXO3A, Antibody
Full Product Name
FOXO3A (Ab-253) Antibody
Product Gene Name
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
FOXO3A (Ab-253) antibody detects endogenous levels of total FOXO3A protein.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
48,406 Da
OMIM
602681
3D Structure
ModBase 3D Structure for O43524
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human FOXO3A around the phosphorylation site of serine 253.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-FOXO3A antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-FOXO3A antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000

Immunohistochemistry (IHC) of anti-FOXO3A antibody

Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3A (Ab-253) antibody.
Anti-FOXO3A Antibody – Cat# MBS857276

Western Blot (WB) of anti-FOXO3A antibody

Western blot analysis of extracts from NIH/3T3 cells treated with using Serum. Left: Using FOXO3A (Ab-253) antibody. Right: Using the same antibody preincubated with synthesized peptide.
Anti-FOXO3A Antibody, Western Blot (WB) – Cat# MBS857276

Product References and Citations for anti-FOXO3A antibody

Li M, Chiu JF, Mossman BT, Fukagawa NK (2006) D J Biol Chem 281, 40429-39 Essafi A, et al. (2005) Oncogene 24, 2317-29 Seoane, J. et al. (2004) Cell 117, 211-223. Brunet A, et al. (2001) Mol Cell Biol 21, 952-65 Brunet A, et al. (1999) Cell 96, 857-68.

Other Notes

Small volumes of anti-FOXO3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXO3A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
UniProt Protein Name
Forkhead box protein O3
UniProt Synonym Protein Names
AF6q21 protein
UniProt Gene Name
FOXO3
NCBI Protein Information
forkhead box protein O3

NCBI Summary for FOXO3A

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for FOXO3A

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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