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MMP14 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8509388

MBS8509388 is an antibody targeting MMP14. Also known as MMP14 (Cleaved-Tyr112) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MMP14, Antibody
Full Product Name
MMP14 (Cleaved-Tyr112) Antibody
Product Gene Name
Product Synonym Names
Matrix metalloproteinase-14 [Precursor]; EC 3.4.24.80; MMP-14; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP-X1; MMP14
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
MMP14 (Cleaved-Tyr112) antibody detects endogenous levels of fragment of activated MMP14 resulting from cleavage adjacent to Tyr112.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
65,894 Da
OMIM
277950
3D Structure
ModBase 3D Structure for P50281
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP14.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MMP14 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MMP14 antibody
WB: 1:500~1:1000
ELISA: 1:20000

Western Blot (WB) of anti-MMP14 antibody

Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 1hour), using MMP14 (Cleaved-Tyr112) antibody.
Anti-MMP14 Antibody – Cat# MBS8509388

Product References and Citations for anti-MMP14 antibody

Sato H., Nature 370:61-65(1994). Takino T., Gene 155:293-298(1995). Okada A., Proc. Natl. Acad. Sci. U.S.A. 92:2730-2734(1995).

Other Notes

Small volumes of anti-MMP14 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP14. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
matrix metalloproteinase-14 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 14
NCBI Official Symbol
MMP14
NCBI Official Synonym Symbols
MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
UniProt Protein Name
Matrix metalloproteinase-14
UniProt Synonym Protein Names
MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP
UniProt Gene Name
MMP14
UniProt Synonym Gene Names
MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
NCBI Protein Information
matrix metalloproteinase-14

NCBI Summary for MMP14

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
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UniProt Comments for MMP14

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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