DIO3 Blocking Peptide – >85% Purity

Name: DIO3 Blocking Peptide; >85% Purity; MyBioSource
Brand: MyBioSource
SKU: MBS823287
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS823287

MBS823287 is a blocking peptide targeting DIO3. Reactive in Human, Mouse, Rat. Host: Synthetic. The peptide is used to block Anti-DIO3 Antibody reactivity. More details

$230 / 1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DIO3, Blocking Peptide

Full Product Name

DIO3 Blocking Peptide

Product Gene Name

DIO3 blocking peptide

Product Synonym Names

ITDI3; TXDI3; Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

Host

Synthetic

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized powder

Purity/Purification

>85%

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Antibody/Peptide Pairs

DIO3 peptide (MBS823287) is used for blocking the activity of DIO3 antibody (MBS821092)

Molecular Weight

33,947 Da

OMIM

601038

Quality Control

The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.

Applications Tested/Suitable for DIO3 blocking peptide

Blocking (BL)

Application Notes for DIO3 blocking peptide

Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.

Other Notes

Small volumes of DIO3 blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DIO3. It may not necessarily be applicable to this product.

NCBI GI #

255958313

NCBI GeneID

1735

NCBI Accession #

NP_001353.4

UniProt Primary Accession #

P55073

UniProt Secondary Accession #

Q8WVN5; G3XAM0

UniProt Related Accession #

P55073

GenBank Nucleotide #

NM_001362.3

NCBI Official Full Name

type III iodothyronine deiodinase

NCBI Official Synonym Full Names

deiodinase, iodothyronine, type III

NCBI Official Symbol

DIO3

NCBI Official Synonym Symbols

D3; 5DIII; TXDI3; DIOIII

UniProt Protein Name

Type III iodothyronine deiodinase

UniProt Synonym Protein Names

5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

Protein Family

Thyroxine 5-deiodinase

UniProt Gene Name

DIO3

UniProt Synonym Gene Names

ITDI3; TXDI3

UniProt Entry Name

IOD3_HUMAN

NCBI Protein Information

type III iodothyronine deiodinase; type 3 DI; type-III 5' deiodinase; type-III 5'-deiodinase; type 3 iodothyronine selenodeiodinase; iodothyronine deiodinase, placental type; thyroxine deiodinase type III (selenoprotein)

NCBI Summary for DIO3

The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

UniProt Comments for DIO3

DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.97.1.11

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: plasma membrane; integral to membrane; endosome membrane

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: thyroid hormone catabolic process; thyroid hormone generation; hormone biosynthetic process; positive regulation of multicellular organism growth

Research Articles on DIO3

1. [review] D3 polymorphisms show no relationship with inter-individual variation in serum thyroid hormone parameters.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DIO3 blocking peptide

Reference Product	PubMed Publications
DLK1 blocking peptide	>97 publications with DIO3 and DLK1
DIO2 blocking peptide	>59 publications with DIO3 and DIO2
GPX4 blocking peptide	>4 publications with DIO3 and GPX4
TRH blocking peptide	>3 publications with DIO3 and TRH

Pathways associated with DIO3 blocking peptide

Products by Pathway	Pathway Diagram
Amine-derived Hormones Pathway antibodies	Amine-derived Hormones Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Regulation Of Thyroid Hormone Activity Pathway antibodies	Regulation Of Thyroid Hormone Activity Pathway Diagram
Selenium Metabolism And Selenoproteins Pathway antibodies	Selenium Metabolism And Selenoproteins Pathway Diagram
Selenium Pathway antibodies	Selenium Pathway Diagram
Thyroid Hormone Signaling Pathway antibodies	Thyroid Hormone Signaling Pathway Diagram
Thyroxine Biosynthesis Pathway antibodies	Thyroxine Biosynthesis Pathway Diagram
Thyroid Hormone Metabolism I (via Deiodination) Pathway antibodies	Thyroid Hormone Metabolism I (via Deiodination) Pathway Diagram
Thyroid Hormone Metabolism I (via Deiodination) Pathway antibodies	Thyroid Hormone Metabolism I (via Deiodination) Pathway Diagram

Diseases associated with DIO3 blocking peptide

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>7 publications with DIO3 and Liver Diseases
Liver Neoplasms Antibodies	>6 publications with DIO3 and Liver Neoplasms
Nervous System Diseases Antibodies	>5 publications with DIO3 and Nervous System Diseases
Hypertrophy Antibodies	>5 publications with DIO3 and Hypertrophy
Heart Diseases Antibodies	>5 publications with DIO3 and Heart Diseases
Prenatal Exposure Delayed Effects Antibodies	>4 publications with DIO3 and Prenatal Exposure Delayed Effects
Carcinoma, Hepatocellular Antibodies	>4 publications with DIO3 and Carcinoma, Hepatocellular
Prenatal Injuries Antibodies	>4 publications with DIO3 and Prenatal Injuries
Fetal Growth Retardation Antibodies	>3 publications with DIO3 and Fetal Growth Retardation
Cell Transformation, Neoplastic Antibodies	>3 publications with DIO3 and Cell Transformation, Neoplastic

Organs/Tissues associated with DIO3 blocking peptide

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>28 publications with DIO3 and Liver
Placenta Antibodies	>14 publications with DIO3 and Placenta
Testis Antibodies	>9 publications with DIO3 and Testis
Uterus Antibodies	>7 publications with DIO3 and Uterus
Adipose Tissue Antibodies	>6 publications with DIO3 and Adipose Tissue
Stomach Antibodies	>3 publications with DIO3 and Stomach
Intestine Antibodies	>1 publications with DIO3 and Intestine