CD137L Human Protein

CHO cells

Human

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

0.45 mg/ml (lot specific)

Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.

A soluble fusion protein consisting of the extracellular domain (184aa) of human CD137 Ligand fused to the extracellular domain (167aa) of murine CD8 alpha.

CHO

Cultured human CEM cells were stimulated 1 day in the presence of 10 ng/ml PMA and 1 muM Ionomycin. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 mul of CD137L-muCD8/Biotin at a concentration of 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a buffer control at a similar concentration. Binding was blocked when reagent was pre incubated 30 minutes with a 10-fold excess of CD137-huIg.

Biotin

Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using affinity and size exclusion chromatography), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by desalting column.

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).