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Ikappab-Alpha (IKBA) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS859574

MBS859574 is an antibody targeting IKBA. Also known as IkappaB-alpha (Ab-305) Antibody. Reactive in Human, Mouse, Rat. More details

$380 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IkappaB-alpha (IKBA), Antibody
Full Product Name
IkappaB-alpha (Ab-305) Antibody
Product Gene Name
Product Synonym Names
I-kappa-B-alpha; IkappaBalpha; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
IkappaB-alpha antibody detects endogenous levels of total IkappaB-alpha protein.
Preparation and Storage
Store at-20 degree C for 1 year.
Molecular Weight
35,609 Da
OMIM
164008
3D Structure
ModBase 3D Structure for P25963
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human IkappaB-alpha.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-IKBA antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-IKBA antibody
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:10000

Testing Data of anti-IKBA antibody

Peptide-+
Anti-IKBA Antibody – Cat# MBS859574

Immunohistochemistry (IHC) of anti-IKBA antibody

Peptide-+ Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using IkappaB-alpha antibody. Immunofluorescence analysis of HuvEc cells, using IkappaB-alpha antibody.
Anti-IKBA Antibody, Immunohistochemistry (IHC) – Cat# MBS859574

Product References and Citations for anti-IKBA antibody

Macdonald M., Cell 72:971-983(1993). Matsuyama N., Genomics 69:72-85(2000). Hillier L.W., Nature 434:724-731(2005).

Other Notes

Small volumes of anti-IKBA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IKBA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NF-kappa-B inhibitor alpha
NCBI Official Synonym Full Names
NFKB inhibitor alpha
NCBI Official Symbol
NFKBIA
NCBI Official Synonym Symbols
IKBA; MAD-3; NFKBI
UniProt Protein Name
NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names
I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
UniProt Gene Name
NFKBIA
UniProt Synonym Gene Names
IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
NCBI Protein Information
NF-kappa-B inhibitor alpha

NCBI Summary for IKBA

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
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UniProt Comments for IKBA

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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