Interleukin 31 (IL-31) ELISA Kit for Mouse

Name: Mouse IL-31 ELISA Kit; Interleukin 31; MyBioSource
Brand: MyBioSource
SKU: MBS044259
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS044259

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IL-31 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-31 antibody to coat Microelisa Stripplate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin 31 (IL-31), ELISA Kit

Full Product Name

Mouse Interleukin 31 ELISA Kit

Product Gene Name

IL-31 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

3.12pg/ml-100pg/ml.

Sensitivity

1.0pg/ml.

Specificity

No significant cross-reactivity or interference between Mouse IL-31 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

18,205 Da

Chromosome Location

Chromosome: 12; NC_000012.11 (122656577..122658746, complement). Location: 12q24.31

OMIM

609509

3D Structure

ModBase 3D Structure for Q6EBC2

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IL-31 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-31 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-31 and IL-31 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of IL-31 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-31 purchase

MBS044259 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 31 (IL-31) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-31. The ELISA analytical biochemical technique of the MBS044259 kit is based on IL-31 antibody-IL-31 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-31 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-31. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-31. It may not necessarily be applicable to this product.

NCBI GI #

62122911

NCBI GeneID

386653

NCBI Accession #

NP_001014358.1

UniProt Primary Accession #

Q6EBC2

UniProt Secondary Accession #

A2RUQ1

UniProt Related Accession #

Q6EBC2

GenBank Nucleotide #

NM_001014336.1

NCBI Official Full Name

interleukin-31

NCBI Official Synonym Full Names

interleukin 31

NCBI Official Symbol

IL31

NCBI Official Synonym Symbols

IL-31

UniProt Protein Name

Interleukin-31

Protein Family

Interleukin-31 receptor

UniProt Gene Name

IL31

UniProt Synonym Gene Names

IL-31

UniProt Entry Name

IL31_HUMAN

NCBI Protein Information

interleukin-31

NCBI Summary for IL-31

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008]

UniProt Comments for IL-31

IL31: Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: immune system process

Research Articles on IL-31

1. IL-31-631 T>G polymorphism was significantly associated with IL-31 blood level but not with disease susceptibility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL-31 elisa kit

Reference Product	PubMed Publications
IL31RA elisa kit	>12 publications with IL-31 and IL31RA
OSMR elisa kit	>9 publications with IL-31 and OSMR
IL6ST elisa kit	>1 publications with IL-31 and IL6ST

Diseases associated with IL-31 elisa kit

Disease Name	Pubmed Publications
Dermatitis, Atopic Antibodies	>34 publications with IL-31 and Dermatitis, Atopic
Inflammation Antibodies	>18 publications with IL-31 and Inflammation
Brain Diseases Antibodies	>3 publications with IL-31 and Brain Diseases
Autoimmune Diseases Antibodies	>2 publications with IL-31 and Autoimmune Diseases
Necrosis Antibodies	>2 publications with IL-31 and Necrosis
Movement Disorders Antibodies	>1 publications with IL-31 and Movement Disorders
Hypersensitivity, Delayed Antibodies	>1 publications with IL-31 and Hypersensitivity, Delayed