Il-3 (IL3) ELISA Kit for Human

Principle of the assay: human IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-3 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-3 amount of sample captured in plate.
Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, ischemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors thatregulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 Themouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the longarm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 aminoacids with the molecular mass of 15KDa.

Small volumes of IL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS175788 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-3 (IL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL3. The ELISA analytical biochemical technique of the MBS175788 kit is based on IL3 antibody-IL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).