L-asarinin Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

354.36

DMSO: Soluble
Chloroform: Soluble
Dichloromethane: Soluble
Ethyl Acetate: Soluble
Acetone: Soluble
Chloroform, Dichloromethane, Ethyl Acetate, Acetone, etc.: Soluble
(<1mg/ml refers to the product slightly soluble or insoluble)

C20H18O6

C5O[C@@H](c1ccc2OCOc2c1)[C@H]6CO[C@H](c3ccc4OCOc4c3)[C@@H]56

133-05-1

Others

Samples containing either URB602 (300uM), MGL (1.4 pM), or both URB602 and MGL are incubated at 37 degree C for 30 min in assay buffer. At various time points, the reaction is stopped with an equal volume of ice-cold methanol and directly analyzed in positive ionization mode by LC/MS. A SB-CN column (150x2.1mM i.d., 5uM) eluted is used with a linear gradient of methanol in water containing 0.25% acetic acid and 5mM ammonium acetate (from 60% to 100% of methanol in 8 min) at a flow rate of 0.5mL/min with column temperature at 50 degree C. Capillary voltage is set at 4 kV and fragmentor voltage is 100V. Nebulizer pressure is set at 60psi. N2 is used as drying gas at a flow rate of 13 liters/min and a temperature of 350 degree C. ESI is in the positive mode and a full scan spectrum is acquired from m/z 100 to 600. Extracted ion chromatograms are used to quantify URB602 ([M+H]+, m/z 296)[2].